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Review
. 2016 Aug 30:7:1359.
doi: 10.3389/fmicb.2016.01359. eCollection 2016.

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists

Praveen Rahi  1 , Om Prakash  1 , Yogesh S Shouche  1
Affiliations
Review

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists

Praveen Rahi et al. Front Microbiol. .

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) based biotyping is an emerging technique for high-throughput and rapid microbial identification. Due to its relatively higher accuracy, comprehensive database of clinically important microorganisms and low-cost compared to other microbial identification methods, MALDI-TOF MS has started replacing existing practices prevalent in clinical diagnosis. However, applicability of MALDI-TOF MS in the area of microbial ecology research is still limited mainly due to the lack of data on non-clinical microorganisms. Intense research activities on cultivation of microbial diversity by conventional as well as by innovative and high-throughput methods has substantially increased the number of microbial species known today. This important area of research is in urgent need of rapid and reliable method(s) for characterization and de-replication of microorganisms from various ecosystems. MALDI-TOF MS based characterization, in our opinion, appears to be the most suitable technique for such studies. Reliability of MALDI-TOF MS based identification method depends mainly on accuracy and width of reference databases, which need continuous expansion and improvement. In this review, we propose a common strategy to generate MALDI-TOF MS spectral database and advocated its sharing, and also discuss the role of MALDI-TOF MS based high-throughput microbial identification in microbial ecology studies.

Keywords: MALDI-TOF mass spectrometry; culturomics; high-throughput identifications; microbial identification; spectral database.

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Figures

Figure 1
Figure 1
Stepwise strategy to develop highly stringent and authentic database for MALDI-TOF MS based microbial identification.
See this image and copyright information in PMC

References

    1. Alatoom A. A., Cunningham S. A., Ihde S. M., Mandrekar J., Patel R. (2011). Comparison of direct colony method versus extraction method for identification of gram-positive cocci by use of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 49, 868–2873. 10.1128/JCM.00506-11 - DOI - PMC - PubMed
    1. Alispahic M., Hummel K., Jandreski-Cvetkovic D., Nöbauer K., Razzazi-Fazeli E., Hess M., et al. . (2009). Species-specific identification and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis. J. Med. Microbiol. 59, 295–301. 10.1099/jmm.0.016576-0 - DOI - PubMed
    1. AlMasoud N., Xu Y., Nicolaou N., Goodacre R. (2014). Optimization of matrix assisted desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the characterization of Bacillus and Brevibacillus species. Anal. Chim. Acta 840, 49–57. 10.1016/j.aca.2014.06.032 - DOI - PMC - PubMed
    1. Almuzara M., Barberis C., Traglia G., Famiglietti A., Ramirez M. S., Vay C. (2015). Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of nonfermenting gram-negative bacilli. J. Microbiol. Methods 112, 24–27. 10.1016/j.mimet.2015.03.004 - DOI - PubMed
    1. Angelakis E., Yasir M., Azhar E. I., Papadioti A., Bibi F., Aburizaiza A. S., et al. . (2014). MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia. BMC Res. Notes 7:892. 10.1186/1756-0500-7-892 - DOI - PMC - PubMed