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. 2016:1496:31-9.
doi: 10.1007/978-1-4939-6463-5_3.

Imaging Golgi Outposts in Fixed and Living Neurons

Mariano Bisbal  1   2   3 , Gonzalo Quassollo  1   2   3 , Alfredo Caceres  4   5   6
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Imaging Golgi Outposts in Fixed and Living Neurons

Mariano Bisbal et al. Methods Mol Biol. 2016.

Abstract

Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.

Keywords: Confocal microscopy; Cultures; Dendrites; Golgi outposts; Live imaging; Neurons.

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