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. 2016 Sep 15;11(9):e0162103.
doi: 10.1371/journal.pone.0162103. eCollection 2016.

Characteristics Analysis of F1 Hybrids between Genetically Modified Brassica napus and B. rapa

Soo-In Sohn  1 , Young-Ju Oh  2 , Kyeong-Ryeol Lee  1 , Ho-Cheol Ko  3 , Hyun-Suk Cho  1 , Yeon-Hee Lee  1 , Ancheol Chang  1
Affiliations

Characteristics Analysis of F1 Hybrids between Genetically Modified Brassica napus and B. rapa

Soo-In Sohn et al. PLoS One. .

Abstract

A number of studies have been conducted on hybridization between transgenic Brassica napus and B. rapa or backcross of F1 hybrid to their parents. However, trait changes must be analyzed to evaluate hybrid sustainability in nature. In the present study, B. rapa and transgenic (BrAGL20) B. napus were hybridized to verify the early flowering phenomenon of F1 hybrids, and F1 hybrid traits were analyzed to predict their impact on sustainability. Flowering of F1 hybrid has been induced slightly later than that of the transgenic B. napus, but flowering was available in the greenhouse without low temperature treatment to young plant, similar to the transgenic B. napus. It is because the BrAGL20 gene has been transferred from transgenic B. napus to F1 hybrid. The size of F1 hybrid seeds was intermediate between those of B. rapa and transgenic B. napus, and ~40% of F1 pollen exhibited abnormal size and morphology. The form of the F1 stomata was also intermediate between that of B. rapa and transgenic B. napus, and the number of stomata was close to the parental mean. Among various fatty acids, the content of erucic acid exhibited the greatest change, owing to the polymorphism of parental FATTY ACID ELONGASE 1 alleles. Furthermore, F2 hybrids could not be obtained. However, BC1 progeny were obtained by hand pollination of B. rapa with F1 hybrid pollen, with an outcrossing rate of 50%.

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Conflict of interest statement

The Institute for Future Environmental Ecology Co., Ltd., does not alter our adherence to all PLOS ONE policies on sharing and materials.

Figures

Fig 1
Fig 1. Scanning electron microscope of pollen grains from Brassica napus, B. rapa, transgenic (TG) B. napus, and the F1 hybrid.
Column 1, pollen sacs; column 2, pollen grains; column 3, archopyle; column 4, enlarged image of pollen grains; column 5, pollen grain surface.
Fig 2
Fig 2. Scanning electron microscope of the adaxial and the abaxial leaf surfaces of Brassica napus, B. rapa, transgenic (TG) B. napus, and F1 hybrids. Bar indicates 50 μm.
The two lower rows show enlarged images of two upper rows.
Fig 3
Fig 3. Real-time RT-PCR quantification of (A) BrAGL20, (B) AGL24, (C) LFY, and (D) AP1 expression in Brassica napus, B. rapa, transgenic (TG) B. napus, and F1 hybrid apical meristems collected over six weeks.
Values indicate the mean (n = 3) accumulation of each gene transcript, relative to the corresponding actin gene levels.
Fig 4
Fig 4. Erucic acid content (%) of Brassica napus, B. rapa, transgenic (TG) B. napus, and F1 hybrid seeds at 10, 20, 30, 40, and 50 days after pollination.
Fig 5
Fig 5. Nucleotide sequence comparison of BnA8.FAE1 and BnC3.FAE1 from Brassica napus, B. rapa, transgenic (TG) B. napus, and F1 hybrids.
See this image and copyright information in PMC

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