Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Nov 15;214(10):1605-1608.
doi: 10.1093/infdis/jiw432. Epub 2016 Sep 16.

A Prophage in Diabetic Foot Ulcer-Colonizing Staphylococcus aureus Impairs Invasiveness by Limiting Intracellular Growth

Jean-Philippe Rasigade  1   2 , Catherine Dunyach-Rémy  3   4 , Anaïs Sapin  1 , Nourredine Messad  3 , Sophie Trouillet-Assant  1 , Céline Dupieux  1   2 , Jean-Philippe Lavigne  3   4 , Frédéric Laurent  1   5   2
Affiliations

A Prophage in Diabetic Foot Ulcer-Colonizing Staphylococcus aureus Impairs Invasiveness by Limiting Intracellular Growth

Jean-Philippe Rasigade et al. J Infect Dis. .

Abstract

The mechanisms that drive the transition from commensality to invasiveness in Staphylococcus aureus are poorly understood. We recently reported that >50% of S. aureus isolates from uninfected diabetic foot ulcers in French patients harbor a prophage, ROSA-like, that is absent from invasive isolates from diabetic foot infections, including osteomyelitis. Here we show that the ROSA-like insertion abolishes the ability of S. aureus to replicate within osteoblasts, the bone-forming cells, greatly reducing damage to infected cells. These results unravel an important mechanism by which particular S. aureus strains are maintained in a commensal state in diabetic foot ulcers.

Keywords: ROSA phage; bacterial intracellular growth; bacterial invasion; diabetic foot infection; osteoblast; osteomyelitis.

PubMed Disclaimer

Publication types