Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016:2016:6273817.
doi: 10.1155/2016/6273817. Epub 2016 Aug 25.

The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativa L.) Oil

Nameer Khairullah Mohammed  1 , Mohd Yazid Abd Manap  2 , Chin Ping Tan  3 , Belal J Muhialdin  3 , Amaal M Alhelli  3 , Anis Shobirin Meor Hussin  2
Affiliations

The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativa L.) Oil

Nameer Khairullah Mohammed et al. Evid Based Complement Alternat Med. 2016.

Abstract

The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

PubMed Disclaimer

Figures

Figure 1
Figure 1
GC-MS chromatography analysis of Nigella sativa oil extracted by (a) 474 supercritical fluid and (b) cold press.
Figure 2
Figure 2
Thymoquinone concentration of Nigella sativa oil as determined by HPLC: (a) 490 supercritical fluid extraction, (b) cold press extraction, and (c) thymoquinone standard.
Figure 3
Figure 3
FTIR spectra of Nigella sativa L. oil scanned at 4,000–650 cm−1: supercritical fluid (SFE) and cold press (CP).
Figure 4
Figure 4
Differential scanning calorimetry (DSC) thermal behavior of Nigella sativa oil: 529 (a) supercritical fluid and (b) cold press.
Figure 5
Figure 5
TGA and DTG curves of weight loss of Nigella sativa oil: (a) supercritical fluid 540 and (b) cold press.
See this image and copyright information in PMC

References

    1. Burits M., Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research. 2000;14(5):323–328. doi: 10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q. - DOI - PubMed
    1. Randhawa M. A., Alghamdi M. S. Anticancer activity of Nigella sativa (Black Seed)—a review. American Journal of Chinese Medicine. 2011;39(6):1075–1091. doi: 10.1142/s0192415x1100941x. - DOI - PubMed
    1. Piras A., Rosa A., Marongiu B., et al. Chemical composition and in vitro bioactivity of the volatile and fixed oils of Nigella sativa L. extracted by supercritical carbon dioxide. Industrial Crops and Products. 2013;46:317–323. doi: 10.1016/j.indcrop.2013.02.013. - DOI
    1. Ramadan M. F. Nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa L.): an overview. International Journal of Food Science and Technology. 2007;42(10):1208–1218. doi: 10.1111/j.1365-2621.2006.01417.x. - DOI
    1. Ramadan M. F., Kroh L. W., Mörsel J.-T. Radical scavenging activity of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.), and Niger (Guizotia abyssinica Cass.) crude seed oils and oil fractions. Journal of Agricultural and Food Chemistry. 2003;51(24):6961–6969. doi: 10.1021/jf0346713. - DOI - PubMed