Quantitative mass spectrometry: an overview

Abstract

Mass spectrometry (MS) is a mainstream chemical analysis technique in the twenty-first century. It has contributed to numerous discoveries in chemistry, physics and biochemistry. Hundreds of research laboratories scattered all over the world use MS every day to investigate fundamental phenomena on the molecular level. MS is also widely used by industry-especially in drug discovery, quality control and food safety protocols. In some cases, mass spectrometers are indispensable and irreplaceable by any other metrological tools. The uniqueness of MS is due to the fact that it enables direct identification of molecules based on the mass-to-charge ratios as well as fragmentation patterns. Thus, for several decades now, MS has been used in qualitative chemical analysis. To address the pressing need for quantitative molecular measurements, a number of laboratories focused on technological and methodological improvements that could render MS a fully quantitative metrological platform. In this theme issue, the experts working for some of those laboratories share their knowledge and enthusiasm about quantitative MS. I hope this theme issue will benefit readers, and foster fundamental and applied research based on quantitative MS measurements.This article is part of the themed issue 'Quantitative mass spectrometry'.

Keywords: bioanalysis; calibration; chemical analysis; mass spectrometry; quantification.

Figure 1.

Electrospray interface for mass spectrometry (a), and its application in quantitative analysis of gramicidin S in methanol–water solution (b). Adapted with permission from [13]. Copyright \copyrightlogo (1985) American Chemical Society.