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Review
. 2016 Oct 28;374(2079):20150370.
doi: 10.1098/rsta.2015.0370.

Mass spectrometry as a quantitative tool in plant metabolomics

Tiago F Jorge  1 , Ana T Mata  1 , Carla António  2
Affiliations
Review

Mass spectrometry as a quantitative tool in plant metabolomics

Tiago F Jorge et al. Philos Trans A Math Phys Eng Sci. .

Abstract

Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided.This article is part of the themed issue 'Quantitative mass spectrometry'.

Keywords: analytical recoveries; mass spectrometry; matrix effects; metabolite quantification; method validation; plant metabolomics.

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Figures

Figure 1.
Figure 1.
Mass spectrometry-based plant metabolomics is a powerful tool to study the metabolic mechanisms underlying plant responses to adverse environmental conditions (abiotic stress) and/or plant–attacker interactions (biotic stress) that adversely affect plant growth and development.
Figure 2.
Figure 2.
Typical plant metabolomics workflow: from experimental design and sample preparation to MS-based metabolite analysis.
Figure 3.
Figure 3.
Parameters required for the analytical validation of MS-based methods for quantitative plant metabolomics experiments.
See this image and copyright information in PMC

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