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. 2016 Sep 15;13(9):724-9.
doi: 10.7150/ijms.15951. eCollection 2016.

Adding Biotin to Parenteral Nutrition Solutions Without Lipid Accelerates the Growth of Candida albicans

Takashi Kuwahara  1 , Shinya Kaneda  1 , Kazuyuki Shimono  1
Affiliations

Adding Biotin to Parenteral Nutrition Solutions Without Lipid Accelerates the Growth of Candida albicans

Takashi Kuwahara et al. Int J Med Sci. .

Abstract

Background: We have previously demonstrated that Candida albicans requires multivitamins (MVs) or lipid to increase rapidly in parenteral nutrition (PN) solutions. In this study, in detail, the effects of vitamins on the growth of C. albicans in PN solutions without lipid were investigated.

Methods: In the 1st experiment, a commercial PN solution without lipid was supplemented with water-soluble vitamins (SVs: vitamins B1, B2, B6, B12 and C, folic acid, nicotinamide, biotin and panthenol), water-insoluble vitamins (IVs: vitamins A, D, E and K) or both (MVs). In the 2nd experiment, the test solutions were prepared by supplementing the PN solution with one of each or all of the SVs. In the 3rd experiment, another commercial peripheral PN (PPN) solution without lipid was supplemented with SVs, nicotinic acid, biotin or both nicotinic acid and biotin. In each of the experiments, a specified number of C. albicans organisms was added to each test solution, and all of the test solutions were allowed to stand at room temperature (23-26ºC). The number of C. albicans was counted at 0, 24, 48 and 72 hours after the addition of the organism.

Results: In the 1st experiment, the C. albicans increased rapidly in the PN solution supplemented with the SVs, but increased slowly without the SVs, regardless of the addition of the IVs. In the 2nd experiment, the C. albicans increased rapidly in the PN solution supplemented with the SVs or biotin, but increased slowly with each of the other water-soluble vitamins. In the 3rd experiment, the C. albicans increased rapidly in the PPN solution supplemented with the SVs or biotin, but increased slowly with the addition of nicotinic acid.

Conclusions: These results suggested that adding MVs or SVs to PN solutions without lipid promotes the growth of C. albicans, and that this effect is mostly attributable to biotin.

Keywords: Candida albicans; biotin; blood stream infection.; microbial growth; parenteral nutrition; vitamin.

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Conflict of interest statement

TK, SK and KS are employees for Otsuka Pharmaceutical Factory, Inc.

Figures

Figure 1
Figure 1
Effects of water-soluble vitamins (SVs), water-insoluble vitamins (IVs) or both (MVs) on the growth of Candida albicans in a PN solution without lipid (CPN).
Figure 2
Figure 2
Effects of 9 individual water-soluble vitamins (SVs) on the growth of Candida albicans in a PN solution without lipid (CPN). a. The effects of vitamins B1, B2, B6, B12 or C on the growth of the N-6 clinical isolate. b. The effects of folic acid, nicotinic acid, biotin or panthenol on the growth of N-6. c. The effects of vitamins B1, B2, B6, B12 or C on the growth of the N-8 clinical isolate. d. The effects of folic acid, nicotinic acid, biotin or panthenol on the growth of N-8.
Figure 3
Figure 3
Effects of biotin or nicotinic acid on the growth of Candida albicans in a PPN solution (CPPN).
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