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. 1989 Jun 15;260(3):785-8.
doi: 10.1042/bj2600785.

Single-step purification and h.p.l.c. analysis of glutathione transferase 8-8 in rat tissues

D J Meyer  1 , E Lalor, B Coles, A Kispert, P Alin, B Mannervik, B Ketterer
Affiliations

Single-step purification and h.p.l.c. analysis of glutathione transferase 8-8 in rat tissues

D J Meyer et al. Biochem J. .

Abstract

GSSG selectively elutes two GSH transferases from a mixture of rat GSH transferases bound to a GSH-agarose affinity matrix. One is a form of GSH transferase 1-1 and the other is shown to be GSH transferase 8-8. By using tissues that lack this form of GSH transferase 1-1 (e.g. lung), GSH transferase 8-8 may thus be purified from cytosol in a single step. Quantitative analysis of the tissue distribution of GSH transferase 8-8 was obtained by h.p.l.c.

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