Review of the fluid flow within intervertebral discs - How could in vitro measurements replicate in vivo?

Abstract

By maintaining a balance between external mechanical loads and internal osmotic pressure, fluid content of intervertebral discs constantly alters causing fluctuations in disc hydration, height, diameter and pressure that govern disc temporal response. This paper reviews and discusses the relevant findings of earlier studies on the disc fluid flow with the aim to understand and remedy discrepancies between in vivo and in vitro observations. New results of finite element model studies are also exploited in order to help identify the likely causes for such differences and underlying mechanisms observed in vitro. In vivo measurements of changes in spinal height and disc fluid content/pressure via stadiometry, magnetic resonance imaging and intradiscal pressure measurements have been carried out. They have demonstrated that the disc volume, fluid content, height and nucleus pressure alter depending to a large extent on prior-current external load conditions. Although the diurnal loading lasts on average nearly twice longer than the subsequent resting (16 vs. 8h), the disc completely recovers its height and volume during the latter period through fluid inflow. In view of much longer periods required to recover disc height and pressure in vitro in ovine, porcine, caprine, bovine and rat discs, concerns have been raised on the fluid inflow through the endplates that might be hampered by clogged blood vessels post mortem. Analyses of discrepancies in the flow-dependent recoveries in vivo and in vitro highlight an excessive fluid content in the latter as a likely cause. To replicate in vivo conditions as closely as possible in vitro, preparation and preconditioning of specimens and/or pressure and osmolarity of the culture media in which specimens are immersed should hence be designed in a manner as to diminish disc hydration level and/or fluid transport.

Keywords: Creep; Disc Hydration; Discrepancies; Flow; Fluid Content; In Vitro; In Vivo; Intervertebral Disc; Recovery.