Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Aug;9(8):919-21.
doi: 10.14202/vetworld.2016.919-921. Epub 2016 Aug 30.

Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction

Raghavendra Prasad Mishra  1 , Udit Jain  1 , Rakesh Kumar Singh  2
Affiliations

Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction

Raghavendra Prasad Mishra et al. Vet World. 2016 Aug.

Abstract

Aim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance.

Materials and methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR).

Results: All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1 , stx2 , eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations.

Conclusion: Genes such as stx1 , stx2 , eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health.

Keywords: deer; polymerase chain reaction; verocytotoxic Escherichia coli.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Agarose gel showing PCR amplified product for VTEC gens isolates from faecal sample of Deer. Lane 1:100bp DNA Ladder, Lane 2: Stx1, Lane 3: Stx2 and hlyA, Lane 5: stx1 and eaeA, Lane 6: Stx2, eaeA and hlyA, Lane 7: Stx2, Lane 9: eaeA and hlyA.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Kumar A, Verma A.K, Sharma A.K, Rahal A. Presence of extended spectum beta-lactamases producing alpha hemolytic Escherichia coli in yellow-wattled lapwing (Vanellus malabaricus) Asian J. Anim. Sci. 2013;7(2):64–69.
    1. Mailk S, Kumar A, Verma A.K, Gupta M.K, Sharma S.D, Sharma A.K, Rahal A. Incidence and drug resistance pattern of collibacillosis in cattle and buffalo calves in Western Uttar Pradesh in India. J. Anim. Health Prod. 2013;1(1):15–19.
    1. Dhama K, Rajagunalan S, Chakraborty S, Verma A.K, Kumar A, Tiwari R, Kapoor S. Food-borne pathogens of animal origin diagnosis, prevention and control and their zoonotic significance - A review. Pak. J. Biol. Sci. 2013;16(20):1076–1085. - PubMed
    1. Kumar A, Verma A.K, Malik S, Gupta M.K, Sharma A, Rahal A. Occurrence of extended spectrum beta-lactamases producing alpha hemolytic Escherichia coli in Neonatal diarrhea. Pak. J. Biol. Sci. 2014;17(1):109–113. - PubMed
    1. Anita Kumar A, Verma A.K, Gupta M.K, Rahal A. Multi drug resistant pathogenic Escherichia coli in water sources and Yamuna river in and around Mathura, India. Pak. J. Biol. Sci. 2014;17(4):540–544. - PubMed

LinkOut - more resources