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. 2016 Aug;9(8):919-21.
doi: 10.14202/vetworld.2016.919-921. Epub 2016 Aug 30.

Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction

Raghavendra Prasad Mishra  1 , Udit Jain  1 , Rakesh Kumar Singh  2
Affiliations

Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction

Raghavendra Prasad Mishra et al. Vet World. 2016 Aug.

Abstract

Aim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance.

Materials and methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR).

Results: All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1 , stx2 , eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations.

Conclusion: Genes such as stx1 , stx2 , eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health.

Keywords: deer; polymerase chain reaction; verocytotoxic Escherichia coli.

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Figures

Figure 1
Figure 1
Agarose gel showing PCR amplified product for VTEC gens isolates from faecal sample of Deer. Lane 1:100bp DNA Ladder, Lane 2: Stx1, Lane 3: Stx2 and hlyA, Lane 5: stx1 and eaeA, Lane 6: Stx2, eaeA and hlyA, Lane 7: Stx2, Lane 9: eaeA and hlyA.
See this image and copyright information in PMC

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