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. 2016 Sep 8;5(1):1515.
doi: 10.1186/s40064-016-3194-1. eCollection 2016.

Molecular identification of human enteroviruses associated with aseptic meningitis in Yunnan province, Southwest China

Yanju Zhu  1 , Xi Zhou  2 , Jiansheng Liu  3 , Longhui Xia  3 , Yue Pan  3 , Junying Chen  3 , Na Luo  3 , Jianzhong Yin  2 , Shaohui Ma  3
Affiliations

Molecular identification of human enteroviruses associated with aseptic meningitis in Yunnan province, Southwest China

Yanju Zhu et al. Springerplus. .

Abstract

Human enteroviruses (EVs) are the major causative agents of aseptic meningitis. In this study, a total of 524 children were admitted to the children Kunming hospital (continental China) for aseptic meningitis manifestations in 2009 and 2010. An EV infection was diagnosed in 85/524 children (16.2 %) and the viruses detected were assigned to 16 serotypes. Most serotypes belonged to the enterovirus B species. Echovirus 9 was predominant (24.7 %), followed by coxsackievirus B5 (23.5 %) and then echovirus 30 (16.5 %). Echovirus 9 was firstly identified as the predominant serotype in sporadic aseptic meningitis which occurred in Yunnan, Southwest China. This work indicates the need to perform large-scale surveillance to gain a better insight into the epidemiology of enteroviruses associated with aseptic meningitis in China.

Keywords: Coxsackievirus B5; Echovirus 30; Echovirus 9; Genotyping; Phylogenetic analysis.

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Figures

Fig. 1
Fig. 1
Phylogenetic trees based on the VP1 gene sequences (906 bp) of E-9 generated by the neighbor-joining algorithm implemented in MEGA (version 6.1) using the Kimura two-parameter substitution model and 1000 bootstrap pseudo-replicates. Only strong bootstrap values (>75 %) are shown. Filled triangle strains isolated in this investigation. Filled circle China
Fig. 2
Fig. 2
Phylogenetic trees based on the VP1 coding sequences (849 bp) of CV-B5 generated by the neighbor-joining algorithm implemented in MEGA (version 6.1) using the Kimura two-parameter substitution model and 1000 bootstrap pseudo-replicates. Only strong bootstrap values (>75 %) are shown. Filled triangle strains isolated in this investigation. Filled circle China
Fig. 3
Fig. 3
Phylogenetic trees based on the VP1 coding sequences (876 bp) of E-30 generated by the neighbor-joining algorithm implemented in MEGA (version 6.1) using the Kimura two-parameter substitution model and 1000 bootstrap pseudo-replicates. Only strong bootstrap values (>75 %) are shown. Filled triangle strains isolated in this investigation. Filled circle China
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