Photochemical characterization of actinorhodopsin and its functional existence in the natural host

Abstract

Actinorhodopsin (ActR) is a light-driven outward H⁺ pump. Although the genes of ActRs are widely spread among freshwater bacterioplankton, there are no prior data on their functional expression in native cell membranes. Here, we demonstrate ActR phototrophy in the native actinobacterium. Genome analysis showed that Candidatus Rhodoluna planktonica, a freshwater actinobacterium, encodes one microbial rhodopsin (RpActR) belonging to the ActR family. Reflecting the functional expression of RpActR, illumination induced the acidification of the actinobacterial cell suspension and then elevated the ATP content inside the cells. The photochemistry of RpActR was also examined using heterologously expressed RpActR in Escherichia coli membranes. The purified RpActR showed λ_max at 534nm and underwent a photocycle characterized by the very fast formation of M intermediate. The subsequent intermediate, named P₆₂₀, could be assigned to the O intermediate in other H⁺ pumps. In contrast to conventional O, the accumulation of P₆₂₀ remains prominent, even at high pH. Flash-induced absorbance changes suggested that there exists only one kind of photocycle at any pH. However, above pH7, RpActR shows heterogeneity in the H⁺ transfer sequences: one first captures H⁺ and then releases it during the formation and decay of P₆₂₀, while the other first releases H⁺ prior to H⁺ uptake during P₆₂₀ formation.

Keywords: Actinorhodopsin; Flash photolysis; Light-driven proton pump; Microbial rhodopsin; Photocycle; Phototrophy.