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. 2016 Sep 23;13(1):160.
doi: 10.1186/s12985-016-0620-5.

Identification of a novel canine parvovirus type 2c in Taiwan

Shu-Yun Chiang  1 , Hung-Yi Wu  2 , Ming-Tang Chiou  1   3 , Min-Chen Chang  1 , Chao-Nan Lin  4   5
Affiliations

Identification of a novel canine parvovirus type 2c in Taiwan

Shu-Yun Chiang et al. Virol J. .

Abstract

Background: Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan.

Methods: During January 2014 to April 2016, fecal or rectal swab samples from 99 dogs with suspected CPV-2 infection in Taiwan were collected. Eighty-eight were identified as being either CPV-2a, -2b or -2c variants positive by real-time PCR and sequence analysis.

Results: Sequence analysis of the 88 isolates confirmed CPV-2c as the dominant variant (54.6 %), followed by CPV-2b (26.1 %) and CPV-2a (19.3 %). Phylogenetic analysis demonstrated that the recent CPV-2c variants are similar to the Chinese CPV-2c strain but can be considered as novel Asian CPV-2c isolates.

Conclusion: The present study provides evidence for the existence of a novel CPV-2c variant in Taiwan.

Keywords: CPV-2c; Canine parvovirus; Taiwan.

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Figures

Fig. 1
Fig. 1
Distribution of CPV-2 variants in Taiwan according to month between January 2014 and April 2016
Fig. 2
Fig. 2
Geographical distribution of CPV-2 variants collected from the dog population between January 2014 and April 2016
Fig. 3
Fig. 3
Phylogenetic analysis of nucleotide sequences of the partial VP2 genes of CPV-2 strains (522 bp). The phylogenetic tree was constructed using the maximum likelihood method with bootstrap analysis (n = 1,000) to determine the best fitting tree. CPV variants are indicated by □, ●, ○, and ▲ for CPV-2, 2a, 2b, and 2c, respectively. The gray diagram represents the Taiwanese CPV-2 variants in this study
See this image and copyright information in PMC

References

    1. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L. Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol. 2001;82:3021–5. doi: 10.1099/0022-1317-82-12-3021. - DOI - PubMed
    1. Martella V, Decaro N, Buonavoglia C. Evolution of CPV-2 and implication for antigenic/genetic characterization. Virus Genes. 2006;33:11–3. doi: 10.1007/s11262-005-0034-8. - DOI - PubMed
    1. Lopez de Turiso JA, Cortes E, Ranz A, Garcia J, Sanz A, Vela C, Casal JI. Fine mapping of canine parvovirus B cell epitopes. J Gen Virol. 1991;72(Pt 10):2445–56. doi: 10.1099/0022-1317-72-10-2445. - DOI - PubMed
    1. Chang SF, Sgro JY, Parrish CR. Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. J Virol. 1992;66:6858–67. - PMC - PubMed
    1. Aldaz J, Garcia-Diaz J, Calleros L, Sosa K, Iraola G, Marandino A, Hernandez M, Panzera Y, Perez R. High local genetic diversity of canine parvovirus from Ecuador. Vet Microbiol. 2013;166:214–9. doi: 10.1016/j.vetmic.2013.06.012. - DOI - PubMed

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