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. 2016 Sep 26:6:33816.
doi: 10.1038/srep33816.

MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin

Yi-Hao Yen  1 , Chao-Min Huang  2 , Kuo-Liang Wei  3 , Jing-Houng Wang  1 , Sheng-Nan Lu  1 , Chuan-Mo Lee  1 , Chao-Hung Hung  1 , Chien-Hung Chen  1 , Po-Lin Tseng  1 , Kuo-Chin Chang  1 , Ming-Chao Tsai  1 , Ming-Tsung Lin  1 , Cheng-Kun Wu  1 , Cheng-Hong Yang  4 , Sin-Hua Moi  4 , Chung-Lung Cho  2 , Tsung-Hui Hu  1
Affiliations

MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin

Yi-Hao Yen et al. Sci Rep. .

Abstract

It has been demonstrated that microRNA-122 (miR-122) plays key roles in the modulation of hepatitis B virus (HBV) replication. This study examined the role of miR-122 in patients with hepatitis C virus (HCV)-HBV dual infection with active hepatitis C who received pegylated interferon-α and ribavirin dual therapy. We enrolled 121 patients with HCV-HBV dual infection after dual therapy. Stored serum was collected before treatment. RT-PCR was used to analyze miR-122. HBsAg seroclearance was noted in 37 (30.1%) cases during a median follow-up period of 5.4 years. miR-122 was significantly lower in HBsAg seroclearance patients than in non-HBsAg seroclearance patients (P < 0.014). Multivariate analysis showed that miR-122 was an independent factor of HBsAg seroclearance (OR: 0.30, 95% CI: 0.09-0.98, P = 0.046). miR-122 was significantly higher in patients who were qHBsAg > 100 IU/mL versus ≤100 IU/mL (P < 0.001). We concluded that in patients with HBV-HCV dual infection with active hepatitis C, miR-122 was associated with HBsAg seroclearance after therapy and qHBsAg level before therapy, indicating that miR-122 plays key roles in modulating HBV replication.

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Figures

Figure 1
Figure 1. Kaplan–Meier analysis of independent factors associated with HBsAg seroclearance.
qHBsAg: quantitative hepatitis B surface antigen.
Figure 2
Figure 2. Kaplan–Meier analysis of independent factors associated with HBsAg seroclearance.
The relative expression levels of serum miR-122 were calculated using a ΔCt method where, ΔCt = Ct(miR-16) – Ct(miR-122). The cycle threshold (C T value) is defined as the number of cycles required for the fluorescent signal to cross the threshold in qPCR, inversely correlates with the miRNA level.
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