Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Sep;171(9):4987-91.
doi: 10.1128/jb.171.9.4987-4991.1989.

Transformation of the archaebacterium Halobacterium volcanii with genomic DNA

S W Cline  1 , L C Schalkwyk, W F Doolittle
Affiliations

Transformation of the archaebacterium Halobacterium volcanii with genomic DNA

S W Cline et al. J Bacteriol. 1989 Sep.

Abstract

We describe optimization of a transformation system for the halophilic archaebacterium Halobacterium volcanii. Transformation of spheroplasts in the presence of polyethylene glycol permits the uptake and expression of high-molecular-weight linear fragments of genomic DNA as well as plasmid or bacteriophage DNA. Transformations can be performed with either fresh or frozen cell preparations. Auxotrophic mutants were transformed to prototrophy with genomic DNA from wild-type cells with efficiencies of 5 x 10(4)/micrograms of DNA and frequencies of 8 x 10(-5) per regenerated spheroplast. The overall efficiency of transformation with genomic DNA implies that genetic recombination is an efficient process in H. volcanii.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1973 Jul 31;124(1):1-10 - PubMed
    1. J Bacteriol. 1985 Apr;162(1):461-2 - PubMed
    1. Methods Enzymol. 1980;65(1):347-53 - PubMed
    1. J Mol Biol. 1968 Oct 14;37(1):157-78 - PubMed
    1. J Cell Biol. 1968 Aug;38(2):337-57 - PubMed

Publication types

LinkOut - more resources