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. 2012 Oct 12;23(3):70-5.
eCollection 2012 Oct.

Miniaturization for Point-of-Care Analysis: Platform Technology for Almost Every Biomedical Assay

Soeren Schumacher, Dorian Sartorius, Eva Ehrentreich-Förster, Frank F Bier

Miniaturization for Point-of-Care Analysis: Platform Technology for Almost Every Biomedical Assay

Soeren Schumacher et al. EJIFCC. .

Abstract

Platform technologies for the changing need of diagnostics are one of the main challenges in medical device technology. From one point-of-view the demand for new and more versatile diagnostic is increasing due to a deeper knowledge of biomarkers and their combination with diseases. From another point-of-view a decentralization of diagnostics will occur since decisions can be made faster resulting in higher success of therapy. Hence, new types of technologies have to be established which enables a multiparameter analysis at the point-of-care. Within this review-like article a system called Fraunhofer ivD-platform is introduced. It consists of a credit-card sized cartridge with integrated reagents, sensors and pumps and a read-out/processing unit. Within the cartridge the assay runs fully automated within 15-20 minutes. Due to the open design of the platform different analyses such as antibody, serological or DNA-assays can be performed. Specific examples of these three different assay types are given to show the broad applicability of the system.

Keywords: DNA; Lab-on-a-Chip; Miniaturization; antibody; antigen.

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Figures

Figure 1
Figure 1
Picture of the credit-card sized ivd-cartridge with integrated optical sensor.
Figure 2
Figure 2
Left: Spotting layout for a multiparameter analysis, Right: False-colored picture of an assay with CRP present as analyte.
Figure 3
Figure 3
Concentration dependency of CRP binding and corresponding cross-reactivity towards other antibodies (BIO I – BIO IV).
Figure 4
Figure 4
Peptide assay specificity assessed by different antibodies specific against the described targets (Reprinted from (9), License number 2982331327162).
Figure 5
Figure 5
Results of a on-chip helicase-dependent amplification of two pathogens (Reprinted from (11), License number 2982330906644).
See this image and copyright information in PMC

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