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. 1989 May 30;490(2):275-84.
doi: 10.1016/s0378-4347(00)82785-1.

High-performance liquid chromatographic determination of tauro- and glyco-conjugated bile acids in human serum

D Labbé  1 , M F Gerhardt, A Myara, C Vercambre, F Trivin
Affiliations

High-performance liquid chromatographic determination of tauro- and glyco-conjugated bile acids in human serum

D Labbé et al. J Chromatogr. .

Abstract

A method for the identification and individual determination of the ten tauro- and glyco-conjugated bile acids is described. It consists in a specific three-step extraction from small serum samples (500 microliters), high-performance liquid chromatographic separation and direct spectrophotometric detection at 119 nm. Extraction can be checked by the use of an internal standard. The reproducibility, recovery and separation of fractions were satisfactory.

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