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. 1989 Jul;27(7):1667-70.
doi: 10.1128/jcm.27.7.1667-1670.1989.

Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains

M D Miliotis  1 , J E Galen, J B Kaper, J G Morris Jr
Affiliations

Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains

M D Miliotis et al. J Clin Microbiol. 1989 Jul.

Abstract

A 24-base oligonucleotide probe specific for a region of the Yersinia enterocolitica virulence plasmid (pYV) associated with HEp-2 cell cytotoxicity and the Sereny reaction was constructed by using sequences flanking critical TnphoA insertions in a subcloned fragment of pYV. This probe, highly specific and sensitive for virulent yersiniae, detected pathogenic Y. enterocolitica isolates in artificially inoculated foods.

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References

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