Fluorescence (Multiwave) Confocal Microscopy

Abstract

In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the depiction of certain structures, like epithelial tumors, nerves, and glands.

Keywords: Fluorescence confocal microscopy; Fluorescent dyes; Fluorophores; Photobleaching; Quenching; Selective featuring of target structures; Staining.