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. 2016 Oct 2:22:3514-3522.
doi: 10.12659/msm.897147.

Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion

Chun-Yan Wang  1 , Shu-Guang Wang  2 , Jia-Li Wang  3 , Li-Ying Zhou  4 , Hong-Jun Liu  5 , Yi-Feng Wang  1
Affiliations

Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion

Chun-Yan Wang et al. Med Sci Monit. .

Abstract

BACKGROUND The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA). MATERIAL AND METHODS Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 RSA patients as the case group and another 142 normal pregnancy women as the control group. We used denaturing high-performance liquid chromatography (DHPLC) to determine the genotypes and allele frequencies of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A. RESULTS The GG genotype and G allele frequencies of miRNA-27a rs895819 A/G were higher in the case group than in the control group, and the AA genotype and A allele frequencies of Leptin rs7799039 G/A were also higher in the case group than in the control group (all P<0.05). MiRNA-27a rs895819 A/G and Leptin rs7799039 G/A polymorphisms increased the risk of RSA (Exp (B)=2.732, 95% CI=1.625~4.596, P=0.000; Exp (B)=4.081, 95% CI=1.817~9.164, P=0.001). GG-AA or AG-AA carriers had a higher risk of RSA. The miRNA-27a expression of AA carriers of miRNA-27a rs895819 was lower than that of AG+GG carriers both in the case and control groups (all P=0.024). The plasma leptin concentration of GG carriers was lower than that of GA+AA carriers in the case group (P=0.026). CONCLUSIONS The polymorphisms of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A may contribute to an increased risk of RSA.

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Figures

Figure 1
Figure 1
(A–C) Denaturing high-performance liquid chromatography (DHPLC) for miRNA-27a rs895819 A/G and sequencing of polymerase chain reaction (PCR) products.
Figure 2
Figure 2
(A–C) Denaturing high-performance liquid chromatography (DHPLC) for Leptin rs7799039 G/A and sequencing of polymerase chain reaction (PCR) products.
Figure 3
Figure 3
The expression of plasma miRNA-27a influenced by miRNA-27a rs895819 A/G and the level of plasma leptin influenced by Leptin rs7799039 G/A. (A) Refers to the expression of plasma miRNA-27a; * refers to P<0.05 when AA genotype was compared with AG + GG genotype; (B) Refers to the level of plasma leptin; * refers to P<0.05 when GG genotype was compared with AG + AA genotype.
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