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. 2016 Oct-Dec;8(4):281-286.
doi: 10.4103/0974-8490.186578.

Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy

Gaurav M Doshi  1 , Vivek V Nalawade  2 , Aaditi S Mukadam  3 , Pratip K Chaskar  2 , Sandeep P Zine  2 , Rakesh R Somani  4 , Hemant D Une  5
Affiliations

Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy

Gaurav M Doshi et al. Pharmacognosy Res. 2016 Oct-Dec.

Abstract

Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants.

Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species.

Materials and methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS.

Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments.

Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.

Summary: Quercetin and rutin were identified from CC roots, PL leaves and BH seeds by liquid chromatography-mass spectroscopy.Quercetin was characterized at (m/z 301) and rutin (m/z 610) as the parent ion peaks.Analogues such as quercetin-O-hexoside, Vicenin 2 and quercetin-3-O-glucoside were identified as fragments.

Keywords: Benincasa hispida; Carissa congesta; Liquid chromatography; Mass spectroscopy; Polyalthia longifolia; Quercetin; Rutin.

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Figures

Figure 1
Figure 1
Mass spectrum showing presence of quercetin (2) in Carissa congesta extract
Figure 2
Figure 2
Mass spectrum showing presence of quercetin (2) and Rutin (3) in Carissa congesta extract
Figure 3
Figure 3
Mass spectrum showing presence of quercetin-O-hexoside (4) in Carissa congesta extract
Figure 4
Figure 4
Mass spectrum showing presence of quercetin-3-O-xyloside/arabinoside (5) in Carissa congesta extract
Figure 5
Figure 5
Mass spectrum showing presence of quercetin (2) in Polyalthia longifolia extract
Figure 6
Figure 6
Mass spectrum showing presence of rutin (3) in Polyalthia longifolia extract
Figure 7
Figure 7
Mass spectrum showing presence of Vicenin 2 (6) and quercetin-3-O-glucoside (7) in Polyalthia longifolia extract
Figure 8
Figure 8
Mass spectrum showing presence of quercetin-O-(O-galloyl)-hexoside (8) in Polyalthia longifolia extract
Figure 9
Figure 9
Mass spectrum showing presence of quercetin (2) in Benincasa hispida extract
Figure 10
Figure 10
Mass spectrum showing presence of quercetin (2) and rutin (3) in Benincasa hispida extract
None
Gaurav M. Doshi
See this image and copyright information in PMC

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