Draft genome sequence of Microbacterium oleivorans strain Wellendorf implicates heterotrophic versatility and bioremediation potential

Abstract

Microbacterium oleivorans is a predominant member of hydrocarbon-contaminated environments. We here report on the genomic analysis of M. oleivorans strain Wellendorf that was isolated from an indoor door handle. The partial genome of M. oleivorans strain Wellendorf consists of 2,916,870 bp of DNA with 2831 protein-coding genes and 49 RNA genes. The organism appears to be a versatile mesophilic heterotroph potentially capable of hydrolysis a suite of carbohydrates and amino acids. Genomic analysis revealed metabolic versatility with genes involved in the metabolism and transport of glucose, fructose, rhamnose, galactose, xylose, arabinose, alanine, aspartate, asparagine, glutamate, serine, glycine, threonine and cysteine. This is the first detailed analysis of a Microbacterium oleivorans genome.

Keywords: Bioremediation potential; Detailed annotation; Draft genome; Metabolic versatility; Microbacterium oleivorans; Student Initiated Microbial Discovery (SIMD) project.

Fig. 1

Negative stain TEM micrograph of Microbacterium oleivorans strain Wellendorf.

Fig. 2

A maximum likelihood phylogenetic tree constructed using multiple sequence alignments of 16S rRNA genes. “Microbacterium oleivorans strain Wellendorf” sequence is shown in bold. GenBank accession numbers are given in parentheses. The tree was obtained under “TN93 + G + I” model with, a proportion of invariable sites of 0.25, and a variable site γ shape parameter of 0.51. The tree was rooted using Escherichia coli partial 16S rRNA gene isolate ECSD9 (not shown). Bootstrap values, in percent, are based on 200 replicates and are shown for branches with > 50% bootstrap support. Multiple sequence alignment, model selection, and maximum likelihood analysis using MEGA .

Fig. 3

(A) COG profile clustering of the genomes compared in this study. (B) Principal component analysis biplot based on the genomic features and COG category distribution in the genomes compared. Genomes are represented by stars (strain names are shown). Strain Wellendorf is shown in blue. Arrows represent genomic features or COG categories used for comparison. The arrow directions follow the maximal abundance, and their lengths are proportional to the maximal rate of change between genomes. The first two components explained 75% of variation.