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. 1989 Jun;217(2-3):324-31.
doi: 10.1007/BF02464900.

Identification and characterization of a locus inhibiting extrachromosomal maintenance of the Streptomyces plasmid SLP1

S R Grant  1 , S C Lee, K Kendall, S N Cohen
Affiliations

Identification and characterization of a locus inhibiting extrachromosomal maintenance of the Streptomyces plasmid SLP1

S R Grant et al. Mol Gen Genet. 1989 Jun.

Abstract

We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. The trans-acting imp function has been localized to a 1.8 kb Eco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of the imp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within the imp region indicate that uninterrupted transcription through all three ORFs is necessary for imp activity.

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References

    1. Mol Gen Genet. 1982;185(2):223-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1428-32 - PubMed
    1. J Bacteriol. 1982 Aug;151(2):668-77 - PubMed
    1. Nature. 1977 Nov 17;270(5634):275-7 - PubMed
    1. Gene. 1984 Apr;28(1):83-91 - PubMed

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