Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Dec;43(12):1681-1692.
doi: 10.1007/s10295-016-1845-4. Epub 2016 Oct 8.

DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis

Yue Zhang  1   2   3 , Chun-Yan Lin  1   2   3 , Xiao-Mei Li  1   2   3 , Zheng-Kun Tang  1   2   3 , Jianjun Qiao  1   2   3 , Guang-Rong Zhao  4   5   6
Affiliations

DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis

Yue Zhang et al. J Ind Microbiol Biotechnol. 2016 Dec.

Abstract

The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S. cinnamonensis. Forward and reverse genetics by overexpression and antisense RNA silence of dasR revealed that DasR positively controls monensin production under nutrient-rich condition. Electrophoresis mobility shift assay (EMSA) showed that DasR protein specifically binds to the promoter regions of both pathway-specific regulatory gene monRII and biosynthetic genes monAIX, monE and monT. Semi-quantitative RT-PCR further confirmed that DasR upregulates the transcriptional levels of these genes during monensin fermentation. Subsequently, co-overexpressed dasR with pathway-specific regulatory genes monRI, monRII or monH greatly improved monensin production.

Keywords: Monensin dasR; Secondary metabolism; Streptomyces cinnamonensis; Transcription factor.

PubMed Disclaimer

References

    1. J Mol Microbiol Biotechnol. 2002 Jul;4(4):417-26 - PubMed
    1. Metab Eng. 2015 May;29:12-25 - PubMed
    1. Curr Opin Microbiol. 2005 Apr;8(2):208-15 - PubMed
    1. Mol Gen Genet. 2000 Nov;264(4):477-85 - PubMed
    1. EMBO Rep. 2008 Jul;9(7):670-5 - PubMed

LinkOut - more resources