The role of galectin-1 in in vitro and in vivo photodynamic therapy with a galactodendritic porphyrin

Abstract

Conventional photodynamic agents used in clinic are porphyrin-based photosensitizers. However, they have low tumour selectivity, which may induce unwanted side-effects and damage to healthy tissues. In this study, we used a porphyrin with dendritic units of galactose (PorGal₈) developed by us, which can target the galactose-binding protein, galectin-1, known to be overexpressed in many tumour tissues. In vitro and in vivo studies had been conducted for the validation of PorGal₈ effectiveness. We showed a specific uptake of PorGal₈ and induction of apoptotic cell death by generating oxidative stress and alterations in the cytoskeleton of bladder cancer cells overexpressing galectin-1. We further validated the photodynamic efficiency of PorGal₈ in athymic nude mice (Balb/c nu/nu) bearing subcutaneously implanted luciferase-positive bladder cancer xenografts, overexpressing galectin-1 protein. PorGal₈ (5 μmol/kg, intraperitoneal), injected 24 h before light delivery (50.4 J/cm²), inhibited tumour growth. We conclude that the use of PorGal₈ enables selective target and cytotoxicity by photodynamic therapy in cancer cells overexpressing galectin-1, preventing undesired phototoxicity in the surrounding healthy tissues.

Keywords: Bladder cancer; Dendritic units of galactose; Galectin-1; Photodynamic therapy; Porphyrin.