LED Blue Light-induced changes in phenolics and ethylene in citrus fruit: Implication in elicited resistance against Penicillium digitatum infection

Abstract

The objective was to investigate whether LED Blue Light (LBL) induces changes in phenolics and ethylene production of sweet oranges, and whether they participate in LBL-elicited resistance against the most important postharvest pathogen (Penicillium digitatum) of citrus fruit. The expression of relevant genes of the phenylpropanoid and ethylene biosynthetic pathways during elicitation of resistance was also determined. Different LBL (wavelength 450nm) quantum fluxes were used within the 60-630μmolm^-2s^-1 range. The HPLC analysis showed that the most relevant increase in phenylpropanoids occurred in scoparone, which markedly increased 3days after exposing fruits to a very high quantum flux (630μmolm^-2s^-1) for 18h. However, phenylpropanoids, including scoparone, were not critical factors in LBL-induced resistance. The genes involved in ethylene biosynthesis were differentially regulated by LBL. Ethylene is not involved in elicited resistance, although high LBL levels increased ethylene production in only 1h.

Keywords: Caffeic acid (PubChem CID: 689043); Chlorogenic acid (PubChem CID: 1794427); Didymin (PubChem CID: 16760075); Diosmin (PubChem CID: 5281613); Eriocitrin (PubChem CID: 83489); Hesperidine (PubChem CID: 3594); Induced resistance; Infection; Isorhoifolin (PubChem CID: 9851181); Isosinesetin (PubChem CID: 632135); Narirutin (PubChem CID: 442431); Nobiletin (PubChem CID: 72344); Phenylalanine ammonia-lyase; Phenylpropanoids; Plant hormones; Postharvest disease; Scoparone (PubChem CID: 8417); Sinensetin (PubChem CID: 145659); Tangeretin (PubChem CID: 68077).