Studies on cis-dichlorodiammineplatinum (II) as a radiosensitizer

Abstract

Cis-dichlorodiammineplatinum (II) (cisDDP) has been extensively studied as an antitumour agent; its binding to DNA has been proved but the radiosensitizing action has scarcely been tested. We report here that on TC.SV -40 mammalian cells cisDPP acts as a dose-modifying factor for ionizing radiation. The radiosensitizing action can be induced by two different mechanisms: reaction with non protein-SH groups and inhibition of repair processes. The cisDDP-DNA complex was studied against increasing radiation doses by analytical centrifugation and by spectrophotometrical measurements. The native complex seems to be more radiation resistant than the denatured one.