Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 May;303(1):79-86.
doi: 10.1007/BF00496188.

Substance P: binding to lipids in the brain

F Lembeck, N Mayer, G Schindler

Substance P: binding to lipids in the brain

F Lembeck et al. Naunyn Schmiedebergs Arch Pharmacol. 1978 May.

Abstract

1. Substance P (SP) could be extracted from brain homogenates with chloroform-methanol by a method which extracts all lipids. 2. SP could be transferred form this total lipid extract (TLE) into an aqueous solution at low pH values (2.0--3.0). 3. At higher pH values (5.5) SP could be transferred from an aqueous phase into an organic phase (chloroform:methanol, 2:1) and recombined with TLE (which was previously freed from endogenous SP) contained in this phase. The binding capacity of TLE for SP exceeded by far the amount of endogenous SP bound originally in the brain extracts. 4. Among the lipids present in TLE, phosphatidylserine was able to bind and release SP in a pH dependent manner. 5. It is suggested that SP bound to phosphatidylserine is the storage form of SP in the brain. The mechanisms by which it is released are still unknown. The possibility that the SP-receptor is also a phospholipid is considered.

PubMed Disclaimer

References

    1. Brain Res. 1976 Jun 11;109(2):285-309 - PubMed
    1. Brain Res. 1977 Feb 25;122(3):534-40 - PubMed
    1. Fed Proc. 1975 Sep;34(10):1922-8 - PubMed
    1. Naunyn Schmiedebergs Arch Pharmakol. 1969;265(3):225-32 - PubMed
    1. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1966;253(2):265-79 - PubMed