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. 2016 Nov;89(11):997-1000.
doi: 10.1002/cyto.a.22997. Epub 2016 Oct 18.

Consistent quantitative gene product expression: #3. Invariance with age

Michael R Loken  1 , Andrew P Voigt  1 , Lisa Eidenschink Brodersen  1 , Wayne Fritschle  1 , Andrew J Menssen  1 , Denise A Wells  1
Affiliations

Consistent quantitative gene product expression: #3. Invariance with age

Michael R Loken et al. Cytometry A. 2016 Nov.

Abstract

The quantitative expression of cell surface antigens and light scattering properties of five cellular reference populations in stressed bone marrow specimens were compared between pediatric and adult patients treated for acute myeloid leukemia (AML). The mean intensity of each antigen as well as the within patient and between patient variability showed striking consistency between the two different age groups. The only difference between the groups of specimens was the proportion of progenitor cells in the adult cohort averaged less than three times the proportion in the pediatric cohort. These data show that the amounts of gene products expressed on bone marrow cells are invariant with age. © 2016 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.

Keywords: adult; bone marrow aspirates; flow cytometry; quantitative antigen expression; support vector machines.

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Figures

Figure 1
Figure 1
Comparison of the intensity of CD34 on the uncommitted progenitor cells between pediatric patients (red) and adult patients (blue). Replicate evaluations (8) for each patient are also displayed. The frequency of the uncommitted progenitor cells for the pediatric and adult groups are depicted as a line on the lower part of the graph, with frequency measurements scaled according to the y‐axis on the right side of the plot.
See this image and copyright information in PMC

References

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