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Meta-Analysis
. 2017 Jan-Mar;48(1):13-24.
doi: 10.1016/j.bjm.2016.08.001. Epub 2016 Sep 30.

Collagenolytic enzymes produced by fungi: a systematic review

Maria Carolina de Albuquerque Wanderley  1 , José Manoel Wanderley Duarte Neto  1 , José Luiz de Lima Filho  1 , Carolina de Albuquerque Lima  2 , José António Couto Teixeira  3 , Ana Lúcia Figueiredo Porto  4
Affiliations
Meta-Analysis

Collagenolytic enzymes produced by fungi: a systematic review

Maria Carolina de Albuquerque Wanderley et al. Braz J Microbiol. 2017 Jan-Mar.

Abstract

Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a deficiency in studies dealing simultaneously with production, characterization and purification of collagenase by fungi. Among microorganisms studied the non-pathogenic fungi Penicillium aurantiogriseum and Rhizoctonia solani stood out in volumetric and specific collagenase activity. The only article found that made sequencing of a true collagenase showed 100% homology with several metalloproteinases fungi. A clear gap in literature about collagenase production by fungi was verified, which prevents further development in the area and increases the need for further studies, particularly full characterization of fungal collagenases with high specificity to collagen.

Keywords: Characterization; Collagenase; Fungus; Production; Purification.

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Figures

Fig. 1
Fig. 1
Collagen molecule: intertwining three alpha chains triple helix.
Fig. 2
Fig. 2
Total articles selected in four different databases using the described methodology.
See this image and copyright information in PMC

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