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. 2016 Jul-Sep;33(3):141-144.
doi: 10.4103/0970-9371.188051.

Comparison of FNAC smears, cytospin smears, and cellblocks of transthoracic guided FNAC of suspected lung tumor: A study of 100 cases

Ankur Singh Kshatriya  1 , Pravina M Santwani  1
Affiliations

Comparison of FNAC smears, cytospin smears, and cellblocks of transthoracic guided FNAC of suspected lung tumor: A study of 100 cases

Ankur Singh Kshatriya et al. J Cytol. 2016 Jul-Sep.

Abstract

Introduction: Transthoracic guided fine needle aspiration cytology (FNAC) of clinically suspected lung tumors is an increasingly common procedure in diagnosis. Cytospin Smear and Cellblock preparations of available material are helpful in subtyping and confirming the diagnosis, and they can also be used for further studies, i.e., special stain and immunohistochemistry, etc.

Aims and objectives: This research was undertaken to study the technique of guided transthoracic lung FNAC of clinically suspected lung tumors and the establish role of FNAC smears, cytospin smears, and cellblocks in the detection and typing of neoplastic lung lesions and correlation.

Materials and methods: Guided FNAC was taken from 100 cases of clinically suspected lung tumor and FNAC smears, cytospin smears, and cellblocks of aspirated material were studied over a period of 2 years from September 2011 to September 2013.

Results: The material adequacies were 80% in FNAC smears, 83% in cytospin smears, and 89% in cellblocks. Additional information supported by cytospin smear and cellblock was 3% and 9%, respectively. Architectural preservation was better in FNAC smears (85%) and cellblocks (73.03%) than that in cytospin smears (31.33%). Morphological preservation was better in FNAC smears (90%) and cellblocks (75.28%) than that in cytospin smears (14.46%). Diagnostic accuracy was increased in the cellblocks and cytospin smears.

Conclusion: Cytospin smear was helpful when low cellular material was obtained, and the concomitant examination of cellblocks not only confirmed the diagnosis of malignancy but also helped in classifying the obtained material and allowed further study on the same.

Keywords: Cellblock; cellblocks; cytospin; cytospin smear; lung tumors; transthoracic fine-needle aspiration cytology (FNAC); transthoracic lung FNAC.

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Figures

Figure 1
Figure 1
FNAC smears: (a) adenocarcinoma (H&E stain, ×400), (b) small cell carcinoma (H&E stain, ×400) cytospin smears: (c) adenocarcinoma (H&E stain, ×400), (d) small cell carcinoma (H&E stain, ×400)
Figure 2
Figure 2
Cellblocks: (a) squamous cell carcinoma (H&E stain, ×400), (b) adenocarcinoma (H&E stain, ×400), (c) small cell carcinoma (H&E stain, ×400), (d) metastatic renal cell carcinoma (H&E stain, ×400)
See this image and copyright information in PMC

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