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. 2016 Dec;17(4):561-571.
doi: 10.1007/s10561-016-9590-5. Epub 2016 Oct 19.

Tissue recovery practices and bioburden: a systematic review

S Brubaker  1 , K Lotherington  2 , Jie Zhao  3 , B Hamilton  4 , G Rockl  5 , A Duong  6 , A Garibaldi  6 , N Simunovic  6 , D Alsop  6 , D Dao  6 , R Bessemer  6 , O R Ayeni  7   8 ; Bioburden Steering Committee and Tissue Recovery Working Group
Collaborators, Affiliations

Tissue recovery practices and bioburden: a systematic review

S Brubaker et al. Cell Tissue Bank. 2016 Dec.

Abstract

For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent processing, tissues can become contaminated. Effective tissue recovery methods, such as minimizing recovery times (<24 h after death) and the number of experienced personnel performing recovery, are examples of factors that can affect the rate of tissue contamination at recovery. Additional factors, such as minimizing the time after asystole to recovery and the total time it takes to perform recovery, the type of recovery site, the efficacy of the skin prep performed immediately prior to recovery of tissue, and certain technical recovery procedures may also result in control of the rate of contamination. Due to the heterogeneity of reported recovery practices and experiences, it cannot be concluded if the use of other barriers and/or hygienic precautions to avoid contamination have had an effect on bioburden detected after tissue recovery. Qualified studies are lacking which indicates a need exists for evidence-based data to support methods that reduce or control bioburden.

Keywords: Allograft; Allograft contamination; Tissue processing; Tissue recovery.

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Conflict of interest statement

Compliance with ethical standardsConflict of interestNo conflicts-of-interest were identified on disclosure forms completed by members of the Committee.Bioburden Steering Committee Membership ListAmber Appleby B.Sc. (Canadian Blood Services), Scott Brubaker (American Association of Tissue Banks), Jeannie Callum, MD, FRCPC (Sunnybrook Health Sciences Centre Blood and Tissue Bank), Graeme Dowling, MD (Comprehensive Tissue Centre), Ted Eastlund, MD (Eastlund Consulting), Margaret Fearon, MD, FRCPC (Canadian Blood Services), Marc Germain, MD, PhD (Héma-Québec), Cynthia Johnston, BSc (Regional Tissue Bank), Ken Lotherington (Canadian Blood Services), Ken McTaggart, MSc (Canadian Blood Services), Jim Mohr, MBA (Canadian Blood Services), Jutta Preiksaitis, MD (Provincial Laboratory for Public Health), Michael Strong, PhD (University of Washington), Martell Winters (Nelson Laboratories), Kimberly Young, MSc (Canadian Blood Services), and Jie Zhao (Comprehensive Tissue Centre).Tissue Recovery Working GroupScott Brubaker (American Association of Tissue Banks), Ken Lotherington (Canadian Blood Services), Jie Zhao (Comprehensive Tissue Centre), Gary Rockl (Southern Alberta Tissue Program), Brian Hamilton (Musculoskeletal Transplant Foundation).

Figures

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Fig. 1
Summary of search strategy
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References

    1. Armiger LC. Viability studies of human valves prepared for use as allografts. Ann Thorac Surg. 1995;60:S118–S121. doi: 10.1016/0003-4975(95)00217-9. - DOI - PubMed
    1. Balshem H, Helfand M, Schünemann HJ, Oxman AD, Kunz R, Brozek J, et al. GRADE guidelines: 3. Rating the quality of evidence. J Clin Epidemiol. 2011;64(4):401–406. doi: 10.1016/j.jclinepi.2010.07.015. - DOI - PubMed
    1. Bettin D, Harms C, Polster J, Niemeyer T. High incidence of pathogenic microorganisms in bone allografts explanted in the morgue. Acta Orthop. 1998;69(3):311–314. doi: 10.3109/17453679809000937. - DOI - PubMed
    1. Bravo D, Rigley TH, Gibran N, Strong DM, Newman-Gage H. Effect of storage and preservation methods on viability in transplantable human skin allografts. Burns. 2000;26(4):367–378. doi: 10.1016/S0305-4179(99)00169-2. - DOI - PubMed
    1. Campbell DG, Oakeshott RD. Bone allograft banking in South Australia. Aust N Z J Surg. 1995;65(12):865–869. doi: 10.1111/j.1445-2197.1995.tb00578.x. - DOI - PubMed

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