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. 1989 Sep;35(9):1897-900.

Measuring tissue factor (factor III) activity in plasma

C Fukuda  1 , K Iijima, K Nakamura
Affiliations
  • PMID: 2776313

Measuring tissue factor (factor III) activity in plasma

C Fukuda et al. Clin Chem. 1989 Sep.

Abstract

This is a method for measuring tissue factor (TF, Factor III, tissue thromboplastin) activity in plasma by using a chromogenic substrate. As pretreatment, the euglobulin fraction of plasma was prepared by removing endogenous inhibitors and heated at 60 degrees C for 3 min to remove fibrinogen. This allowed us to measure the low TF activity in plasma that could not otherwise be measured. Neither phospholipids nor coagulation factors VII, IX, X, or Xa in the samples interfere. Within-run and day-to-day reproducibility were both good. The mean value obtained by this method for normal persons was 1.02 (SD 0.91) arbitrary units/L. A markedly high plasma TF activity of 20 arb. units/L or more was observed in patients with some types of disseminated intravascular coagulation.

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