Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

Affiliations

¹ Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea; Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla.
² Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada; National Institute for Nanotechnology, National Research Council Canada, Edmonton, Alberta, Canada.
³ Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla.
⁴ Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
⁵ Department of Internal Medicine, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
⁶ Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital and Nano-Bio Regenerative Medical Institute, Hallym University College of Medicine, Chuncheon, Korea. Electronic address: doctordk@naver.com.
⁷ Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla. Electronic address: scho2@health.usf.edu.

PMID: 27771323
PMCID: PMC5467741
DOI: 10.1016/j.jaci.2016.09.026

Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

Dae Woo Kim et al. J Allergy Clin Immunol. 2017 May.

. 2017 May;139(5):1692-1695.e6.

doi: 10.1016/j.jaci.2016.09.026. Epub 2016 Oct 19.

Authors

Affiliations

¹ Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea; Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla.
² Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada; National Institute for Nanotechnology, National Research Council Canada, Edmonton, Alberta, Canada.
³ Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla.
⁴ Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
⁵ Department of Internal Medicine, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
⁶ Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital and Nano-Bio Regenerative Medical Institute, Hallym University College of Medicine, Chuncheon, Korea. Electronic address: doctordk@naver.com.
⁷ Division of Allergy-Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, Fla. Electronic address: scho2@health.usf.edu.

PMID: 27771323
PMCID: PMC5467741
DOI: 10.1016/j.jaci.2016.09.026

No abstract available

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Conflict of interest statement

Conflict of interest: The authors declare that they have no relevant conflicts of interest.

Figures

**Figure 1. Cross-talk between human mast cells and epithelial cells in eosinophilic nasal polyps**
**(A, B)** IHC detection of periostin was performed within the NP tissues from patients with CRSwNP (*eosinophil, asterisk; mast cell, red arrow head; fibroblast, black arrow*). Black scale bar=10μm. **(C)** Representative double immunostaining (red: tryptase and blue: periostin). Arrows indicate double-positive immune cells (x1000). Black scale bar=10μm. **(D)** Counts of double-positive cells in NE-NP and E-NP (n=5 for each group). (E) Periostin mRNA expression was measured by qRT-PCR in LAD2 cells stimulated with IgE, IL-4, IL-13 and TNF-α (n=9 for each group). **(F)** Periostin protein expression stimulated by IgE (n=3 for each group). **(G)** Number of integrin αV-positive epithelial cells per 100 cells was determined and averaged from three different areas of epithelium (n=10 for Control-UP, n=10 for CRSsNP-UP, n=10 for CRSwNP-UP, and n=37 for CRSwNP-NP) in IHC assays of tissue sections. The number of integrin αV-positive inflammatory cells was counted from five of the densest areas (HPF; x400) and averaged for each group. **(H)** Correlation between integrin αV and TSLP at the protein level. **(I)** Correlation between periostin and TSLP at the protein level. **(J)** Periostin-induced TSLP production in NHBE cells after Th2 cytokine and Poly(I:C) treatment (n=3 for each group). **(K, L)** Periostin-induced TSLP production was reversed by anti-integrin αVβ3 (K) and anti- integrin αVβ5 (L) (n=3 for each group). *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001 using the Mann-Whitney U-test.

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References

1. Kim MA, Izuhara K, Ohta S, Ono J, Yoon MK, Ban GY, et al. Association of serum periostin with aspirin-exacerbated respiratory disease. Ann Allergy Asthma Immunol. 2014;113:314–20. - PubMed
1. Kanemitsu Y, Matsumoto H, Izuhara K, Tohda Y, Kita H, Horiguchi T, et al. Increased periostin associates with greater airflow limitation in patients receiving inhaled corticosteroids. J Allergy Clin Immunol. 2013;132:305–12. e3. - PubMed
1. Hanania NA, Wenzel S, Rosen K, Hsieh HJ, Mosesova S, Choy DF, et al. Exploring the effects of omalizumab in allergic asthma: an analysis of biomarkers in the EXTRA study. Am J Respir Crit Care Med. 2013;187:804–11. - PubMed
1. Corren J, Lemanske RF, Hanania NA, Korenblat PE, Parsey MV, Arron JR, et al. Lebrikizumab treatment in adults with asthma. N Engl J Med. 2011;365:1088–98. - PubMed
1. Laury AM, Hilgarth R, Nusrat A, Wise SK. Periostin and receptor activator of nuclear factor kappa-B ligand expression in allergic fungal rhinosinusitis. Int Forum Allergy Rhinol. 2014;4:716–24. - PubMed

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Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

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Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

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