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. 2016:2016:9761697.
doi: 10.1155/2016/9761697. Epub 2016 Sep 28.

Oxidative Stress Markers and Their Dynamic Changes in Patients after Acute Ischemic Stroke

Ingrid Žitňanová  1 , Pavol Šiarnik  2 , Branislav Kollár  2 , Mária Chomová  1 , Petra Pazderová  1 , Lucia Andrezálová  1 , Miriam Ježovičová  1 , Katarína Koňariková  1 , Lucia Laubertová  1 , Zuzana Krivošíková  3 , Laura Slezáková  3 , Peter Turčáni  2
Affiliations

Oxidative Stress Markers and Their Dynamic Changes in Patients after Acute Ischemic Stroke

Ingrid Žitňanová et al. Oxid Med Cell Longev. 2016.

Abstract

We have focused on determining the range of oxidative stress biomarkers and their dynamic changes in patients at different time points after the acute ischemic stroke (AIS). 82 patients with AIS were involved in our study and were tested: within 24 h from the onset of the attack (group A); at 7-day follow-up (group B); and at 3-month follow-up (group C). 81 gender and age matched volunteers were used as controls. Stroke patients in group A had significantly higher concentrations of plasma lipid peroxides and urine 8-isoprostanes when compared with controls. Protein carbonyls were not significantly different in any experimental group compared to controls. Antioxidant capacity of plasma was increased only in experimental group C. Activities of superoxide dismutase and catalase were elevated in all three experimental AIS groups compared to controls. Paraoxonase activity was reduced in groups A and B and unchanged in group C when compared to controls. Glutathione peroxide activity was elevated only in group A. Our results suggest that free radical damage is the highest within 24 h after the attack. During the next 3 months oxidative damage to lipids caused by free radicals is reduced due to activated antioxidant system.

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Figures

Figure 1
Figure 1
Semiquantitative evaluation (a) and representative blot of SOD1 protein expression (b) in lysates of erythrocytes in controls (Co) and ischemic stroke patients. A: patients within 24 h after AIS, B: patients at 7-day follow-up, and C: patients at 3-month follow-up. Data are presented as the means ± SD. n = 6; ∗ P < 0.05; ∗∗ P < 0.01 versus acute ischemic stroke group A.
Figure 2
Figure 2
Semiquantitative evaluation (a) and a representative blot of catalase protein expression (b) in lysates of erythrocytes in controls (Co) and ischemic stroke patients. A: patients within 24 h after AIS, B: patients at 7-day follow-up, and C: patients at 3-month follow-up.
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