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. 2016 Dec;13(12):985-988.
doi: 10.1038/nmeth.4034. Epub 2016 Oct 24.

Bright photoactivatable fluorophores for single-molecule imaging

Jonathan B Grimm  1 , Brian P English  1 , Heejun Choi  1 , Anand K Muthusamy  1 , Brian P Mehl  1 , Peng Dong  1 , Timothy A Brown  1 , Jennifer Lippincott-Schwartz  1 , Zhe Liu  1 , Timothée Lionnet  1 , Luke D Lavis  1
Affiliations

Bright photoactivatable fluorophores for single-molecule imaging

Jonathan B Grimm et al. Nat Methods. 2016 Dec.

Abstract

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

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