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. 2016 Dec:131:110-112.
doi: 10.1016/j.mimet.2016.10.011. Epub 2016 Oct 21.

Does biomass production correlate with metabolic activity in Staphylococcus aureus?

Beatriz Alonso  1 , José Lucio  2 , María Jesús Pérez-Granda  3 , Raquel Cruces  1 , Carlos Sánchez-Carrillo  4 , Emilio Bouza  5 , María Guembe  6
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Does biomass production correlate with metabolic activity in Staphylococcus aureus?

Beatriz Alonso et al. J Microbiol Methods. 2016 Dec.

Abstract

We assessed agreement between the crystal violet binding assay and the XTT assay in the classification of biofilm production in 492 Staphylococcus aureus strains from bacteremic patients. We found that the overall correlation between the procedures was 46.5%. Biomass production and metabolic activity must be assessed simultaneously.

Keywords: Biofilm; Biomass; Crystal violet; Metabolic activity; Staphylococcus aureus; XTT.

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