Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016:2016:9837598.
doi: 10.1155/2016/9837598. Epub 2016 Sep 29.

Sequestration and Distribution Characteristics of Cd(II) by Microcystis aeruginosa and Its Role in Colony Formation

Xiangdong Bi  1 , Ran Yan  2 , Fenxiang Li  3 , Wei Dai  2 , Kewei Jiao  3 , Qixing Zhou  3 , Qi Liu  2
Affiliations

Sequestration and Distribution Characteristics of Cd(II) by Microcystis aeruginosa and Its Role in Colony Formation

Xiangdong Bi et al. Biomed Res Int. 2016.

Abstract

To investigate the sequestration and distribution characteristics of Cd(II) by Microcystis aeruginosa and its role in Microcystis colony formation, M. aeruginosa was exposed to six different Cd(II) concentrations for 10 days. Cd(II) exposure caused hormesis in the growth of M. aeruginosa. Low concentrations of Cd(II) significantly induced formation of small Microcystis colonies (P < 0.05) and increased the intracellular polysaccharide (IPS) and bound extracellular polysaccharide (bEPS) contents of M. aeruginosa significantly (P < 0.05). There was a linear relationship between the amount of Cd(II) sequestrated by algal cells and the amount added to cultures in the rapid adsorption process that occurred during the first 5 min of exposure. After 10 d, M. aeruginosa sequestrated nearly 80% of 0.2 mg L-1 added Cd(II), while >93% of Cd(II) was sequestrated in the groups with lower added concentrations of Cd(II). More than 80% of the sequestrated Cd(II) was bioadsorbed by bEPS. The Pearson correlation coefficients of exterior and interior factors related to colony formation of M. aeruginosa revealed that Cd(II) could stimulate the production of IPS and bEPS via increasing Cd(II) bioaccumulation and bioadsorption. Increased levels of cross-linking between Cd(II) and bEPS stimulated algal cell aggregation, which eventually promoted the formation of Microcystis colonies.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effects of various concentrations of Cd(II) on algal cell density of M. aeruginosa.
Figure 2
Figure 2
Effects of various concentrations of Cd(II) on the inhibition rate of M. aeruginosa.
Figure 3
Figure 3
Effects of Cd(II) on colony formation by M. aeruginosa. Values sharing the same letters are not significantly different, whereas those marked with different letters are significantly different (P < 0.05).
Figure 4
Figure 4
Changes in the proportions of Cd(II) bioadsorbed by bEPS and bioaccumulated inside algal cells and of Cd(II) in the culture medium for M. aeruginosa exposed to various concentrations of Cd(II).
Figure 5
Figure 5
Cd(II) content bioaccumulated inside algal cells. Values sharing the same letters are not significantly different, whereas those marked with different letters are significantly different (P < 0.05).
Figure 6
Figure 6
Cd(II) bioadsorbed by bEPS. Values sharing the same letters are not significantly different, whereas those marked with different letters are significantly different (P < 0.05).
Figure 7
Figure 7
Effects of various concentrations of Cd(II) on the bEPS content of M. aeruginosa (pg/cell). Values sharing the same letters are not significantly different, whereas those marked with different letters are significantly different (P < 0.05).
Figure 8
Figure 8
Effects of various concentrations of Cd(II) on the IPS content of M. aeruginosa (pg/cell). Values sharing the same letters are not significantly different, whereas those marked with different letters are significantly different (P < 0.05).
See this image and copyright information in PMC

References

    1. Zhao L. F., Lu L., Li M., Xu Z. R., Zhu W. Effects of Ca and Mg levels on colony formation and EPS content of cultured M. aeruginosa . Procedia Environmental Sciences B. 2011;10:1452–1458.
    1. Yang Z., Kong F. X., Shi X. L., Zhang M., Xing P., Cao H. S. Changes in the morphology and polysaccharide content of Microcystis aeruginosa (Cyanobacteria) during flagellate grazing. Journal of Phycology. 2008;44(3):716–720. doi: 10.1111/j.1529-8817.2008.00502.x. - DOI - PubMed
    1. Wang W., Liu Y., Yang Z. Combined effects of nitrogen content in media and Ochromonas sp. grazing on colony formation of cultured Microcystis aeruginosa . Journal of Limnology. 2010;69(2):193–198. doi: 10.3274/jl10-69-2-01. - DOI
    1. Gan N. Q., Xiao Y., Zhu L., et al. The role of microcystins in maintaining colonies of bloom-forming Microcystis spp. Environmental Microbiology. 2012;14(3):730–742. doi: 10.1111/j.1462-2920.2011.02624.x. - DOI - PubMed
    1. Yuan H. Z., Shen J., Liu E. F. Assessment and characterization of heavy metals and nutrients in sediments from Taihu lake. Journal of Environmental Sciences-China. 2011;32(3):649–657. - PubMed

MeSH terms

LinkOut - more resources