New fluorescence assay for the quantitation of fungi
- PMID: 2778063
- PMCID: PMC267727
- DOI: 10.1128/jcm.27.9.2003-2007.1989
New fluorescence assay for the quantitation of fungi
Abstract
Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.
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