Hormonal and fitness consequences of behavioral assortative mating in the convict cichlid (Amatitlania siquia)

Abstract

In monogamous species, partner compatibility is a key factor influencing pairing and reproductive success. In pairs with biparental care, studies have mostly focused on behavioral compatibility because it is likely to encourage the coordination of parental care within pairs, leading to a better reproductive success. Behavior modulation, throughout the breeding season and as a function of the social context, is under the regulatory feedback control of endocrine mechanisms. From this link, the idea of hormonal partner compatibility as a key component of pair cohesion and maintenance has recently emerged. Here, we investigated the link between partner behavioral assortment and their hormonal response to the pairing context. We formed assortative and disassortative pairs of convict cichlids based on their behavioral type (proactive or reactive) and took hormone and fitness measurements. Testosterone, 11-ketotestosterone, 17β-estradiol and cortisol levels were measured from fish-holding water before and after pair formation. We found no relationship between the behavioral type of individuals and their pre-pairing hormone levels. Only the level of cortisol was affected by the partner but independently of its behavioral type. Reproductive success was not affected by the level of hormonal similarity within pairs, but we found that the variation in 11-ketotestosterone similarity between the isolated context and the pairing context was related to spawning size, and the variation in cortisol similarity to the number of fry. Behavioral compatibility does not reflect hormonal compatibility in the convict cichlid, but the relationship between reproductive success and the flexibility of hormonal similarity between partners suggests hormonal adjustment within pairs in this species.

Keywords: Amatitlania siquia; Assortative mating; Behavioral type; Fish; Partner compatibility; Steroid hormones.