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. 2016 Feb 13;9(8):e30593.
doi: 10.5812/jjm.30593. eCollection 2016 Aug.

Prevalence of Virulence-Related Determinants in Clinical Isolates of Staphylococcus epidermidis

Shahin Najar Peerayeh  1 , Ali Jazayeri Moghadas  2 , Mehrdad Behmanesh  3
Affiliations

Prevalence of Virulence-Related Determinants in Clinical Isolates of Staphylococcus epidermidis

Shahin Najar Peerayeh et al. Jundishapur J Microbiol. .

Abstract

Background: Staphylococcus epidermidis, a member of the human flora, is recognized as an opportunistic pathogen and cause of nosocomial infections. Staphylococcus epidermidis surface components are able to establish bacteria on the host surface, and cause infection.

Objectives: The frequency of icaA, IS256, aap, fbe and bhp in clinical isolates of S. epidermidis were investigated in this study.

Materials and methods: Fifty-nine S. epidermidis isolates were collected from blood (50), wound (1), urine (4) and tracheal (4) samples (Tehran, Iran). Staphylococcus epidermidis isolates were identified with conventional bacteriological tests. Virulence-associated genes were detected by specific polymerase chain reactions (PCRs).

Results: Of the 59 S. epidermidis, fbe was found in 89.8%, while aap and bhp were observed in 64.4% and 15.3% of the samples, respectively. Coexistence of aap and fbe was found in 32 isolates, while coexistence of bhp and fbe was observed in five isolates. Two isolates were negative for the investigated genes.

Conclusions: Prevalence of fbe and aap was significantly different from similar studies, yet frequency of bhp was in accordance with other studies. Prevalence of icaA and IS256 was not significantly different from some studies while a significant difference was observed when results were compared with some other studies.

Keywords: Staphylococcal Infections; Staphylococcus epidermidis; Virulence Factors.

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Figures

Figure 1.
Figure 1.. Polymerase Chain Reaction for icaA
1 - 3, Positive clinical strains; 4, positive control (215 bp); 5, marker (100 bp).
Figure 2.
Figure 2.. Polymerase Chain Reaction for IS256
1, Marker (100 bp); 2, positive control (251 bp); 3 - 6, positive clinical strains.
Figure 3.
Figure 3.. Polymerase Chain Reaction for aap
1, Marker (100 bp); 2, positive control (465 bp); 3, negative control; 4 - 9, positive clinical strains.
Figure 4.
Figure 4.. Polymerase Chain Reaction for fbe
1, Marker (100 bp); 2, S. epidermidis strain k28 (positive control, 273 bp); 3 - 5, positive clinical strains.
Figure 5.
Figure 5.. Polymerase Chain Reaction for bhp
1, Marker (100 bp); 2, positive control (1548 bp); 3 and 4, positive clinical strains.
See this image and copyright information in PMC

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