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. 2016 Aug 31;9(9):e30000.
doi: 10.5812/jjm.30000. eCollection 2016 Sep.

Antibacterial Efficacy of Super-Oxidized Water on Enterococcus faecalis Biofilms in Root Canal

Recai Zan  1 , Tayfun Alacam  2 , Ihsan Hubbezoglu  3 , Tutku Tunc  4 , Zeynep Sumer  4 , Oguzhan Alici  3
Affiliations

Antibacterial Efficacy of Super-Oxidized Water on Enterococcus faecalis Biofilms in Root Canal

Recai Zan et al. Jundishapur J Microbiol. .

Abstract

Background: The success of endodontic treatment depends on a few crucial factors. One of these factors is the complete chemomechanic preparation of root canal against various bacteria. In particular, the effect of resistant bacteria may cause intense pain with flare-up and formation of periapical lesions. Therefore, the strong effect of irrigants plays an important role in terms of the complete elimination of these bacteria to achieve long-term successful treatment.

Objectives: The aim of this study was to investigate the antibacterial effects of super-oxidized water (SPO) in root canals infected with Enterococcus faecalis biofilms.

Methods: One hundred twenty single-root, premolar teeth were selected. Initially, the teeth were prepared and then disinfected. E. faecalis were inoculated and kept at 37°C for 24 hours in the root canals. The re-inoculation procedure was repeated on the first, fourth, seventh, and tenth days. The infected root canals were divided into one negative (saline) and one positive (sodium hypochlorite) control group and four experimental groups (super-oxidized water: 1, 2, 3, or 5 minutes) (n = 20). Paper points were placed in the root canals to control and evaluate the biofilm formation. Biofilms were counted on blood agar plates, and data was evaluated and statistically analyzed using one-way ANOVA and Tukey's test.

Results: Although sodium hypochlorite (NaOCl) showed no statistically significant difference when compared with three and five minutes of SPO irrigation (P > 0.05), NaOCl showed statistically significant differences among all other groups (P < 0.05).

Conclusions: Super-oxidized water indicated a remarkable and similar bactericidal effect to that of traditional NaOCl against E. faecalis biofilms. In terms of successful endodontic treatment approaches, super-oxidized water may be used as an effective irrigation solution in clinics.

Keywords: Bacteria; Biofilm; Disinfection; Super-Oxidized Water.

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Figures

Figure 1.
Figure 1.. Stereomicroscopic Image of Developed E. faecalis Biofilm in a Root Canal
Figure 2.
Figure 2.. Stereomicroscopic Image of Remaining Bacteria in Group 2
Figure 3.
Figure 3.. Stereomicroscopic Image of Remaining Bacteria in Group 3
See this image and copyright information in PMC

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