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. 2016 Nov 3;17(1):864.
doi: 10.1186/s12864-016-3206-0.

Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

Tobias Eisenberg  1 , Ahmad Fawzy  2   3   4 , Werner Nicklas  5 , Torsten Semmler  6 , Christa Ewers  4
Affiliations

Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

Tobias Eisenberg et al. BMC Genomics. .

Abstract

Background: The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.

Results: Our data provide a novel and reliable basis of support for previously established phylogeny from this group and give a deeper insight into characteristics of genome structure and gene functions. Full genome analyses revealed that most S. moniliformis strains under study form a heterogeneous population without any significant clustering. Analysis of infra-species variability for this highly pathogenic rat bite fever organism led to the detection of three specific variable number tandem analysis loci with high discriminatory power.

Conclusions: This highly useful and economical tool can be directly employed in clinical samples without laborious prior cultivation. Our and prospective case-specific data can now easily be compared by using a newly established MLVA database in order to gain a better insight into the epidemiology of this presumably under-reported zoonosis.

Keywords: Fingerprinting; Leptotrichiaceae; Multi locus variable number tandem repeat analysis (MVLA); Next generation sequencing; Phylogeny; Streptobacillus; Typing.

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Figures

Fig. 1
Fig. 1
Maximum likelihood phylogenetic tree of the genus Streptobacillus (strains 1–29 according to Table 1). The tree is based on 281 orthologous genes including 57,841 SNPs
Fig. 2
Fig. 2
Unrooted maximum likelihood phylogenetic tree of 23 Streptobacillus moniliformis strains from this study. The tree is based on 775 orthologous genes including 5,211 SNPs
See this image and copyright information in PMC

References

    1. Gaastra W, Boot R, Ho HT, Lipman LJ. Rat bite fever. Vet Microbiol. 2009;133(3):211–28. doi: 10.1016/j.vetmic.2008.09.079. - DOI - PubMed
    1. Eribe ER, Paster BJ, Caugant DA, Dewhirst FE, Stromberg VK, Lacy GH, Olsen I. Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov. Int J Syst Evol Microbiol. 2004;54(Pt 2):583–92. doi: 10.1099/ijs.0.02819-0. - DOI - PubMed
    1. Harwich MD, Jr, Serrano MG, Fettweis JM, Alves JM, Reimers MA, Buck GA, Jefferson KK. Genomic sequence analysis and characterization of Sneathia amnii sp. nov. BMC Genomics. 2012;13(Suppl 8):S4. doi: 10.1186/1471-2164-13-S8-S4. - DOI - PMC - PubMed
    1. Levaditi C, Nicolau S, Poincloux P. Sur le rôle étiologique de Streptobacillus moniliformis (nov. spec.) dans l’érythème polymorphe aigu septicémique. C R Acad Sci. 1925;180:1188–90.
    1. Eisenberg T, Glaeser S, Nicklas W, Mauder N, Contzen M, Aledelbi K, Kämpfer P. Streptobacillus felis sp. nov. isolated from a cat with pneumonia. Int J Syst Evol Microbiol. 2015;65(7):2172–8. doi: 10.1099/ijs.0.000238. - DOI - PubMed

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