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. 2016 Oct 17;4175(4):377-389.
doi: 10.11646/zootaxa.4175.4.7.

Use of DNA barcoding to distinguish the malaria vector Anopheles neivai in Colombia

Andrés López-Rubio  1 , Juan Suaza-Vasco  2 , Paula L Marcet  3 , Natalia Ruíz-Molina  2 , Lorenzo Cáceres  3 , Charles Porter  3 , Sandra Uribe  2
Affiliations

Use of DNA barcoding to distinguish the malaria vector Anopheles neivai in Colombia

Andrés López-Rubio et al. Zootaxa. .

Abstract

A reference 535 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI), acquired from specimens of An. neivai Howard, Dyar & Knab, 1913 from its type locality in Panama, was used as a tool for distinguishing this species from others in the subgenus Kerteszia. Comparisons with corresponding regions of COI between An. neivai and other species in the subgenus (An. bellator Dyar & Knab 1906, An. homunculus Komp 1937, An cruzii Dyar & Knab, 1908 and An. laneanus Corrêa & Cerqueira, 1944) produced K2P genetic distances of 8.3-12.6%, values well above those associated with intraspecific variation. In contrast, genetic distances among 55 specimens from five municipalities in the Colombian Pacific coastal state of Chocó were all within the range of 0-2.5%, with an optimized barcode threshold of 1.3%, the limit for unambiguous differentiation of An. neivai. Among specimens from the Chocó region, 18 haplotypes were detected, two of which were widely distributed over the municipalities sampled. The barcode sequence permits discrimination of An. neivai from sympatric species and indicates genetic variability within the species; aspects key to malaria surveillance and control as well as defining geographic distribution and dispersion patterns.

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Figures

FIGURE 1
FIGURE 1
Collection municipalities map for Anopheles neivai in Colombia (CO) and Panama (PA).
FIGURE 2
FIGURE 2
Genetic differences (K2P) among An. neivai collected specimens (A) and against other species from Kerteszia (B) and Nyssorhynchus from NCBI (C).
FIGURE 3
FIGURE 3
DNA barcode threshold optimization for An. neivai.
FIGURE 4
FIGURE 4
Dendrogram (K2P) for An. neivai specimens from Colombia, Panama, with other Kerteszia and Nyssorhynchus species (based on a 535 bp COI alignment. Branch support was provided by bootstrap resampling (10000 replicates).
FIGURE 5
FIGURE 5
Haplotype network for An. neivai collected in Colombia and Panama (based on a 535 bp COI alignment).
See this image and copyright information in PMC

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