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. 2016 Dec;32(4):514-516.
doi: 10.1007/s12288-016-0692-9. Epub 2016 Jun 10.

Detection of Hemoglobin New York [β113 (G15) Val→Glu, GTG>GAG] in a Thai Woman by Capillary Electrophoresis

Sitthichai Panyasai  1 , Sakorn Pornprasert  2
Affiliations

Detection of Hemoglobin New York [β113 (G15) Val→Glu, GTG>GAG] in a Thai Woman by Capillary Electrophoresis

Sitthichai Panyasai et al. Indian J Hematol Blood Transfus. 2016 Dec.

Abstract

Hemoglobin (Hb) New York [β113 (G15) Val→Glu, GTG>GAG] is a very rare β-chain variant found in Thailand. This variant is often missed by routine laboratory testing because Hb New York and Hb A have the identical retention time on high performance liquid chromatography. We reported here for the first time that the detection of Hb New York in a Thai woman by using capillary electrophoresis (CE). A peak of Hb New York located ahead of Hb A at the electrophoretic zone 11 with a level of 42.8 %. The DNA sequencing revealed the GTG>GAG mutation at codon 113 for Hb New York on one allele of β-globin gene. Therefore, the CE has a high efficiency to prevent the misinterpretation of hemoglobin analysis in patients who are heterozygote of this variant.

Keywords: Capillary electrophoresis; Detection; Hb New York; High performance liquid chromatography; Thai woman.

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Conflict of interest statement

The authors report no conflicts of interest. Informed consent This study was approved by the Ethics Committee of the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. The informed consent was obtained from all individual participants included in the study.

Figures

Fig. 1
Fig. 1
The HPLC chromatogram (a) and CE electrophoregram (b) of the proband
Fig. 2
Fig. 2
Representative DNA sequence derived from the proband
See this image and copyright information in PMC

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