Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Sep-Oct;24(5):423-430.
doi: 10.1590/1678-775720160139.

The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate

Ozan Kuduban  1 , Muhammed Recai Mazlumoglu  2 , Selma Denktas Kuduban  3 , Ertugrul Erhan  4 , Nihal Cetin  5 , Osman Kukula  6 , Oguzhan Yarali  7 , Ferda Keskin Cimen  8 , Murat Cankaya  9
Affiliations

The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate

Ozan Kuduban et al. J Appl Oral Sci. 2016 Sep-Oct.

Abstract

Objective:: To investigate the effect of HRE (Hippophae rhamnoides extract) on oral mucositis induced in rats with MTX.

Material and methods:: Experimental animals were divided into groups as healthy (HG), HRE+MTX (HMTX), and control group, which received MTX (MTXC). HMTX group received 50 mg/kg HRE while MTXC and HG groups received equivolume distilled water with gavage once a day. After one hour of HRE and distilled water administration, HMTX and MTXC groups received a single dose of oral MTX 5 mg/ kg. This procedure was repeated for one month.

Results:: The levels of MDA, IL-1β, and TNF-α were found to be significantly higher in the cheek, lower lip, and tongue tissue of the animals receiving MTX, compared with HG and HMTX groups; however, these parameters were lower in the cheek and low lip tissue, and a milder damage ocurred in these tissues, compared with the tongue tissue in MTXC group. No histopathologic damage was observed in the cheek, lower lip, and tongue tissues of the rats treated with HRE.

Conclusion:: This findings indicate that HRE as a natural product is an important advantage compared with synthetic drugs for prophylaxis of oral mucositis developed due to MTX.

PubMed Disclaimer

Conflict of interest statement

The authors declare no potential conflict of interests regarding the authorship and/or publication of this article.

Figures

Figure 1
Figure 1. a: The effects of methotrexate on malondialdehyde (MDA) levels in the cheek mucosal tissues; b: The effects of methotrexate on tGSH levels in the cheek mucosal tissues; c: The effects of methotrexate on IL-1β gene expression level in the cheek mucosal tissues. *p<0.0001
Figure 2
Figure 2. a: The effects of methotrexate on malondialdehyde (MDA) levels in the lower lip tissues; b: The effects of methotrexate on tGSH levels in the lower lip tissues; c: The effects of methotrexate on IL-1β gene expression level in the lower lip tissues. *p<0.0001
Figure 3
Figure 3. a: The effects of methotrexate on malondialdehyde (MDA) levels in the tongue tissues; b: The effects of methotrexate on tGSH levels in the tongue tissues; c: The effects of methotrexate on IL-1ß gene expression level in the tongue tissues. *p<0.0001
Figure 4
Figure 4. a: Normal histopathologic appearance of the rat cheek tissue of Healthy Group (HG) (HE&40); b: Normal histopathologic appearance of the rat cheek tissue of HG (HE&20); c: Normal histopathologic appearance of the rat cheek tissue of HG (HE&40); d: Dilated congested blood vessels (arrow) and edema (star) are distinguished in the cheek mucosa of MTXC group given methotrexate (MTX) (HEx40); e: Mild edema (star) and dilated congested blood vessels (arrow) are monitored in the lip mucosa of MTXC group given MTX (HEx40); f: Dilated congested blood vessels are seen in the tongue mucosa of MTXC group given MTX (HE&100); g: Fibroblastic proliferation (star) and fat cells replaced muscle layer are monitored in the tongue mucosal tissue of MTXC group given MTX (arrow) (HE&40); h: Dilated, congested, proliferated blood vessels (arrow) are seen in the tongue mucosa of MTXC group given MTX (HE&100); i: Edema (star) and dilated congested blood vessels (arrow) are monitored in the tongue mucosal tissue of MTXC group given MTX (HE&100); j: Appearance of the cheek tissue of HMTX group treated with Hippophae rhamnoides extract (HRE) resembling normal mucosa (HEx10); k: Appearance of the lip tissue of HMTX group treated with HRE resembling normal mucosa (HEx20); l: Appearance of the tongue tissue of HMTX group treated with HRE resembling normal mucosa (HEx100)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Alamir I, Boukhettala N, Aziz M, Breuillé D, Déchelotte P, Coëffier M. Beneficial effects of cathepsin inhibition to prevent chemotherapy-induced intestinal mucositis. Clin Exp Immunol. 2010;162(2):298–305. - PMC - PubMed
    1. Altindag Ö, Küçükoglu B. Intoxication due to high dose methotrexate in a patient with rheumatoid arthritis: a case report. Turk J Rheumatology. 2011;26(1):58–61.
    1. Araújo AA, Borba PB, Souza FH, Nogueira AC, Saldanha TS, Araújo TE, et al. In a methotrexate-induced model of intestinal mucositis, olmesartan reduced inflammation and induced enteropathy characterized by severe diarrhea, weight loss, and reduced sucrose activity. Biol Pharm Bull. 2015;38(5):746–752. - PubMed
    1. Bairati I, Meyer F, Gélinas M, Fortin A, Nabid A, Brochet F, et al. A randomized trial of antioxidant vitamins to prevent second primary cancers in head and neck cancer patients. J Natl Cancer Inst. 2005;97(7):481–488. - PubMed
    1. Blumenthal RD, Lew W, Reising A, Soyne D, Osorio L, Ying Z, et al. Anti-oxidant vitamins reduce normal tissue toxicity induced by radio-immunotherapy. Int J Cancer. 2000;86(2):276–280. - PubMed

Publication types

MeSH terms