Three-dimensional cell culture models for investigating human viruses

doi:10.1007/s12250-016-3889-z

Review

. 2016 Oct;31(5):363-379.

doi: 10.1007/s12250-016-3889-z. Epub 2016 Oct 27.

Three-dimensional cell culture models for investigating human viruses

Bing He¹, Guomin Chen², Yi Zeng³

Affiliations

¹ State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
² State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. guominch2013@163.com.
³ State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. zengyicdc@sina.com.

PMID: 27822716
PMCID: PMC7090760
DOI: 10.1007/s12250-016-3889-z

Review

Three-dimensional cell culture models for investigating human viruses

Bing He et al. Virol Sin. 2016 Oct.

. 2016 Oct;31(5):363-379.

doi: 10.1007/s12250-016-3889-z. Epub 2016 Oct 27.

Authors

Bing He¹, Guomin Chen², Yi Zeng³

Affiliations

¹ State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
² State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. guominch2013@163.com.
³ State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. zengyicdc@sina.com.

PMID: 27822716
PMCID: PMC7090760
DOI: 10.1007/s12250-016-3889-z

Abstract

Three-dimensional (3D) culture models are physiologically relevant, as they provide reproducible results, experimental flexibility and can be adapted for high-throughput experiments. Moreover, these models bridge the gap between traditional two-dimensional (2D) monolayer cultures and animal models. 3D culture systems have significantly advanced basic cell science and tissue engineering, especially in the fields of cell biology and physiology, stem cell research, regenerative medicine, cancer research, drug discovery, and gene and protein expression studies. In addition, 3D models can provide unique insight into bacteriology, virology, parasitology and host-pathogen interactions. This review summarizes and analyzes recent progress in human virological research with 3D cell culture models. We discuss viral growth, replication, proliferation, infection, virus-host interactions and antiviral drugs in 3D culture models.

Keywords: human viruses; scaffolds; three-dimensional (3D) cell culture models.

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References

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[1] Achilli TM, Meyer J, Morgan JR. Advances in the formation, use and understanding of multi-cellular spheroids. Expert Opin Biol Ther. 2012;12:1347–1360. doi: 10.1517/14712598.2012.707181. - DOI - PMC - PubMed

[2] Achilli TM, Meyer J, Morgan JR. Advances in the formation, use and understanding of multi-cellular spheroids. Expert Opin Biol Ther. 2012;12:1347–1360. doi: 10.1517/14712598.2012.707181. - DOI - PMC - PubMed

[3] Aizaki H, Nagamori S, Matsuda M, Kawakami H, Hashimoto O, Ishiko H, Kawada M, Matsuura T, Hasumura S, Matsuura Y, Suzuki T, Miyamura T. Production and release of infectious hepatitis C virus from human liver cell cultures in the three-dimensional radial-flow bioreactor. Virology. 2003;314:16–25. doi: 10.1016/S0042-6822(03)00383-0. - DOI - PubMed

[4] Aizaki H, Nagamori S, Matsuda M, Kawakami H, Hashimoto O, Ishiko H, Kawada M, Matsuura T, Hasumura S, Matsuura Y, Suzuki T, Miyamura T. Production and release of infectious hepatitis C virus from human liver cell cultures in the three-dimensional radial-flow bioreactor. Virology. 2003;314:16–25. doi: 10.1016/S0042-6822(03)00383-0. - DOI - PubMed

[5] Alemany R, Balague C, Curiel DT. Replicative adenoviruses for cancer therapy. Nat Biotechnol. 2000;18:723–727. doi: 10.1038/77283. - DOI - PubMed

[6] Alemany R, Balague C, Curiel DT. Replicative adenoviruses for cancer therapy. Nat Biotechnol. 2000;18:723–727. doi: 10.1038/77283. - DOI - PubMed

[7] Aly HH, Shimotohno K, Hijikata M. 3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV. Biochem Biophys Res Commun. 2009;379:330–334. doi: 10.1016/j.bbrc.2008.12.054. - DOI - PubMed

[8] Aly HH, Shimotohno K, Hijikata M. 3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV. Biochem Biophys Res Commun. 2009;379:330–334. doi: 10.1016/j.bbrc.2008.12.054. - DOI - PubMed

[9] Anacker D, Moody C. Generation of organotypic raft cultures from primary human keratinocytes. J Vis Exp. 2012;60:1–4. - PMC - PubMed

[10] Anacker D, Moody C. Generation of organotypic raft cultures from primary human keratinocytes. J Vis Exp. 2012;60:1–4. - PMC - PubMed

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Three-dimensional cell culture models for investigating human viruses

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Three-dimensional cell culture models for investigating human viruses

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