Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2016 Nov 8:8:53.
doi: 10.1186/s13099-016-0140-2. eCollection 2016.

Viral metagenomics of fecal samples from non-human primates revealed human astrovirus in a chimpanzee, China

Xiaochun Wang  1   2 , Jinxin Wang  1 , Chenglin Zhou  3 , Shixing Yang  1 , Quan Shen  1 , Wen Zhang #  1 , Dunwu Qi #  2
Affiliations
Case Reports

Viral metagenomics of fecal samples from non-human primates revealed human astrovirus in a chimpanzee, China

Xiaochun Wang et al. Gut Pathog. .

Abstract

Background: Human astroviruses (HAstVs) are commonly identified worldwide as important aetiological agents of acute gastroenteritis in all age groups. More and more evidences challenged the paradigm that AstV infections are species-specific. Yet to date, AstVs associated with human infections have not been detected in any animal hosts.

Results: Viral metagenomics methods were used to detect viral nucleic acids in fecal samples from 69 captive non-human primates (NHPs) from three zoos in China. Sequence reads showing high similarity to astrovirus MLB2 were found in feces from a chimpanzee with diarrhea. The complete genome of this astrovirus was determined and deposited in the GenBank under accession number KX273058 (named SAstV-nj). Phylogenetic analysis based on complete genomes revealed that SAstV-nj was closely related to and shared >98% nucleotide sequence identity with the previous human astrovirus MLB2 strains.

Conclusions: This study suggested that MLB2-related astroviruses might have the potential of cross-species transmission between human and NHP.

Keywords: Astrovirus; NHP; Phylogenetic analysis; Viral metagenomics method.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Mapping astroviral reads in the raw library data over the referenced genome of JF742759 (a) and neighbor-joining phylogenetic trees using MEGA6 (b, c). b was the phylogenetic tree based on capsid protein gene sequences. c indicated the phylogenetic tree based on RNA-dependent RNA polymerase gene sequences. Black dot indicated the SAstV-nj identified in this study. GenBank accession numbers were shown
See this image and copyright information in PMC

References

    1. De Benedictis P, Schultz-Cherry S, Burnham A, Cattoli G. Astrovirus infections in humans and animals—molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol. 2011;11(7):1529–1544. doi: 10.1016/j.meegid.2011.07.024. - DOI - PMC - PubMed
    1. Lee MH, Jeoung HY, Park HR, Lim JA, Song JY, An DJ. Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea. Virus Genes. 2013;46(1):175–181. doi: 10.1007/s11262-012-0816-8. - DOI - PMC - PubMed
    1. Karlsson EA, Small CT, Freiden P, Feeroz MM, Matsen FT, San S, Hasan MK, Wang D, Jones-Engel L, Schultz-Cherry S. Non-human primates harbor diverse mammalian and avian astroviruses including those associated with human infections. PLoS Pathog. 2015;11(11):e1005225. doi: 10.1371/journal.ppat.1005225. - DOI - PMC - PubMed
    1. Bosch A, Pinto RM, Guix S. Human astroviruses. Clin Microbiol Rev. 2014;27(4):1048–1074. doi: 10.1128/CMR.00013-14. - DOI - PMC - PubMed
    1. Mendenhall IH, Smith GJ, Vijaykrishna D. Ecological drivers of virus evolution: astrovirus as a case study. J Virol. 2015;89(14):6978–6981. doi: 10.1128/JVI.02971-14. - DOI - PMC - PubMed

Publication types

LinkOut - more resources